DRR-BRS SHARED INSTRUMENTATION GRANT

这是一份为JeoL 1200申请资金的提案。 电子显微镜，以取代过时的飞利浦301。 一 需要高质量电子显微镜的各种研究 作为他们成功的先决条件， 购买新的显微镜。 拟议项目将 研究：a）蛋白质的翻译后分选， 内质网和细胞器，（B）极化 将病毒糖蛋白递送至 上皮细胞，（c）核糖体附着过程的各个方面 （d）内质网的分布和功能 中枢神经系统髓鞘的表达（e） 类固醇分泌细胞的分化和调节，（f） 髓鞘相关糖蛋白在髓鞘形成中的作用 神经，（g）小分子量代谢的作用， 囊性纤维化，（h）细胞生长的合成和分泌 调节因子，（i）补体级联的抑制， (j)疟原虫抗原的表征，（k）表达 角蛋白和膜蛋白在分化过程中 （1）维生素E的摄取和代谢。 上述 项目主要由一些调查人员进行， 在细胞生物学系，多年来， 和谐共享主要设备和其他 部门内的资源。 此外，其他一些用户 将共享这个设施 拟议 显微镜特别适合作为共享仪器。

This is a proposal requesting funds for the purpose of a JeoL 1200 electron microscope to replace an obsolescent Philips 301. A variety of studies, which demand high quality electron microscopy as a prerequisite for their success, will benefit from that acquisition of the new microscope. The proposed projects will investigate: a) the posttranslational sorting of proteins out of the endoplasmic reticulum and to cellular organelles, (b) the polarized delivery of viral glycoproteins to the plasma membranes of epithelial cells, (c) aspects of the process of ribosome attachment to the endoplasmic reticulum, (d) the distribution and functional expression of central nervous system myelin (e) the differentiation and modulation of steroid secreting cells, (f) the role of the myelin associated glycoprotein in myelination of nerves, (g) the role of a small molecular weight metabolic in cystic fibrosis, (h) the synthesis and secretion of cellular growth regulatory factors, (i) the inhibition of the complement cascade, (j) the characterization of plasmodial antigens, (k) the expression of keratins and membrane proteins during differentiation of epithelia, (1) the uptake and metabolism of vitamin E. The above projects are largely being undertaken by a number of investigators in the Department of Cell Biology, who have over the years harmoniously shared major pieces of equipment and other resources within the department. In addition, several other users from outside the department will share the facility. The proposed microscope is especially suited as a shared instrument.