PEPTIDE SYNTHESIZER & AMINO ACID ANALYZER

德克萨斯大学蛋白质测序中心目前 提供氨基酸测序和合成服务。这个 中心拥有Beckman型号890C型旋转杯定序器和 贝克曼型121MB氨基酸分析仪。贝克曼阿尔特斯高中 高效液相色谱仪用于分析PTH- 氨基酸。这些工具正由一种新的 实用477A型高灵敏度气相测序仪 生物系统，根据新的NIH拨款1-SL0-RR03335-01。这 仪器将允许我们在10-500皮摩尔范围内工作。 因此，我们将能够提供蛋白质的NH2末端序列 来自丙烯酰胺凝胶中的单个条带。这是一个应用程序 用于采集两台仪器，一台高灵敏度氨基 酸度分析仪与气相测序仪的灵敏度相匹配， 还有一个多肽合成器。没有氨基酸分析仪的高 德克萨斯大学奥斯汀分校存在敏感性，没有肽 合成器可用于分子生物学 社区。多肽合成器将被用来制造多肽 一组不同的调查人员非常需要。其中一些 这些多肽的用途包括构建 用作抗原和拓扑试剂的多肽用于地图 不同蛋白质特定区域的功能。采办 这些新工具将使该中心能够履行其 目前的功能与最先进的仪器，必要的 敏感度，还将增加一种重要的新功能，多肽 综合。

The Protein Sequencing Center of the University of Texas currently provides an amino acid sequencing and composition service. The Center has a Beckman model 890C spinning cup sequencer and a Beckman model 121MB Amino Acid Analyzer. Beckman Altex high performance liquid chromatography equipment is used to analyze PTH- amino acids. These instruments are being supplemented by a new high sensitivity gas phase sequencer, model 477A, of Applied Biosystems, under a new NIH grant 1-Sl0-RR03335-01. This instrument will allow us to operate in the 10-500 picomole range. Thus we will be able to provide NH2-terminal sequences of proteins from individual bands in acrylamide gels. This is an application for the acquisition of two instruments, a high sensitivity amino acid analyzer to match the sensitivity of the gas phase sequencer, and a peptide synthesizer. No amino acid analyzer of high sensitivity exists on the UT Austin campus and no Peptide Synthesizer is available for use by the molecular biology community. The Peptide Synthesizer will be used to make peptides greatly needed by a diverse group of investigators. Some of the uses to which the peptides will be put include the construction of peptides for use as antigens and as topological reagents to map functions of specific regions of different proteins. Acquisition of these new instruments would enable the Center to perform its present function with state-of-the-art instruments of the necessary sensitivity and would also add an important new capability, peptide synthesis.