BRAIN PEPTIDES ON PITUITARY FUNCTIONS AND DIABETES

更大的计划项目申请的一部分，涉及基本和 应用神经内分泌学在生殖、生长、应激和 糖尿病。具体地说，建议建立老年人的生理学 最近分离出的下丘脑生长激素释放因子，特别是 评估其在胰腺等腺垂体以外的组织中的活性 (回复：胰岛素和胰升糖素的分泌)，肠道(Re。VIP的分泌)， 胃(Re.胃泌素的分泌)。制备抗肾综合征出血热抗血清 设计和验证GRF放射免疫分析方法并建立 免疫组织化学定位GRF。为了合成大量的GRF 适用于正常人和青少年或青少年疾病患者的临床研究 成熟期出现糖尿病和几种类型的发育问题。刻画 合成下丘脑内啡肽-促肾上腺皮质激素释放因子； 鉴定除促肾上腺皮质激素外的几种皮质营养因子，尤其是 控制肾上腺皮质雄激素分泌的因素 (雄激素)。鉴定多种形式的天然生长抑素 中枢神经系统、胰腺和其他外周来源。设计出新的 极高灵敏度的分析和结构化学方法 最终导致将多肽排序在一个范围内的能力 纳米分子。

Part of a larger Program Project application dealing with fundamental and applied neuroendocrinology in the field of reproduction, growth, stress and diabetes. Specifically it is proposed to establish the physiology of the recently isolated hypothalmic growth hormone releasing factor, in particular to assess its activity on tissues other than the adenohypophysis such as pancreas (re. secretion of insulin and glucagon), the gut (re. secretion of VIP), the stomach (re. secretion of gastrin). To produce antisera against GRF for designing and validating a radio immunoassay to GRF and for establishing a mapping of GRF by immunohistochemistry. To synthesize large quantities of GRF for clinical studies in normal individuals and patients with juvenile or maturity onset diabetes and several types of growth problems. To characterize and synthesize the hypothalmic endorphin-corticotropin-releasing-factor; to characterize several corticotrophic factors other than ACTH, in particular a factor controlling the secretion of androgens by the adrenal cortex (androtropin). To characterize multiple forms of native somatostatin from central nervous system, pancreas and other peripheral sources. To devise new methods of analytical and structural chemistry of extremely high sensitivity eventually leading to the ability to sequence polypeptides in the range of one nanomole.