ONTOGENY OF PREPROCHOLECYSTOKININ, PROENKEPHALIN AND TYROSIN HYDROXYLASE IN RATS

脑多肽是一种神经递质或神经调节剂。 成人的大脑。在胚胎大脑中，它们可能在 神经元的组织和发育以及突触发生。我们 本实验研究了大鼠胆囊收缩素(CCK)和脑啡肽的个体发生 通过测量大鼠从出生前12天到成年期的大脑 在相同的组织中，多肽和mRNA的水平是平行的。 CCK免疫反应肽与脑啡肽 免疫活性多肽(脑啡肽-IR)的测定 一种特殊的放射免疫分析方法。信使RNA水平为 用Poly(A)+RNA与 相应的cDNAs探针。我们还研究了其个体发育。 酪氨酸羟基酶(TH)，合成过程中的限制性酶 去甲肾上腺素和多巴胺。单胺，它们的代谢物 并对脑实质中的mRNA水平进行了比较。 发育过程中大鼠的黑质和蓝斑。

Brain peptides are neurotransmitter or neuromodulators in the adult brain. In the embryonic brain, they may play a role in the organization and development of neurons and synaptogenesis. We studied the ontogeny of cholecystokinin (CCK) and enkephalin in the rat brain from day 12 prenatal to adulthood by measuring the peptides and the mRNA levels in parallel in the same tissues. CCK-immunoreactive peptides (CCK-IR) and enkephalin immunoreactive peptides (enkephalin-IR) were measured by using a specific radioimmunoassay. Messenger RNA levels were measured by blot hybridization of poly(A)+ RNA with the corresponding cDNA probes. We also studied the ontogeny of tyrosine hydroxylase (TH), the limiting enzyme in the synthesis of noradrenaline and dopamine. The monoamines, their metabolites and the mRNA levels were measured comparatively in substantia nigra and locus ceruleus of the rat during development.