FLUOROIMMUNOASSAY APPARATUS

具有衰变时间的新型稀土络合荧光探针 比背景衰减时间常数长得多的常数 典型的有机材料提供了巨大的潜力 时间分辨荧光法灵敏度的改进。 然而，如果他们要与放射免疫分析方法竞争， 灵敏度要求变得如此之高，以至于超载恢复 荧光探测器的故障是一个主要问题。广博的过去 在这个问题上的工作在很大程度上不适用于非常低的速度 以及对这种情况的水平要求。因此，首字母 正在努力确定最佳的荧光 检测装置和辅助信号过载限制电路。 与极低荧光探头有关的问题 样本浓度将与疾控中心合作进行研究， 它对这种方法作为艾滋病的另一种工具感兴趣 研究。最后，对激励的细节进行了评价， 将学习光学滤波和数字信号处理。 并进行优化。

New rare earth chelate fluorescent probes with decay time constants much longer than the background decay time-constant of typical organic materials offer the potential of a great improvement in sensitivity of time resolved fluorimetry. However, if they are to rival radioimmunoassay methods the sensitivity requirement becomes so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally, an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.