CYTOSKELETAL PROTEINS IN HUMAN NEOPLASMS

原肌球蛋白和其他细胞骨架蛋白的合成和含量 在各种人类肿瘤细胞系和组织中的作用， 为了确定这些蛋白质的合成是否在人类中受到抑制， 肿瘤，因为它是在NIH/3 T3细胞转化的致癌病毒。 由各种组织类型合成的原肌球蛋白将通过 2-双向聚丙烯酰胺凝胶电泳 重量、等电点、细胞骨架定位、色氨酸缺失 和免疫反应性。 原肌球蛋白在大肠癌细胞中的表达 然后将评估这些类型。 其他细胞骨架的合成 将在相同的制备物中评价蛋白质。 以下系统 将研究：淋巴样细胞，髓样细胞，结肠癌，膀胱癌 癌和乳腺癌。

The synthesis and content of tropomyosins and other cytoskeletal proteins in various human tumor cell lines and tissues will be investigated in order to determine whether synthesis of these proteins is suppressed in human neoplasms as it is in NIH/3T3 cells transformed by oncogenic viruses. Tropomyosins synthesized by various tissue types will be identified by 2-dimensional polyacrylamide gel electrophoresis on the basis of molecular weight, isoelectric point, cytoskeletal localization, absence of tryptophan and immunological reactivity. Expression of tropomyosins in tumor cells of these types will then be evaluated. Synthesis of other cytoskeletal proteins will be evaluated in the same preparations. The following systems will be studied: lymphoid cells, myeloid cells, colon carcinoma, bladder carcinoma, and mammary carcinoma.