GENETIC POLYMORPHISMS AND RELATIONSHIP TO CANCERS OF THE RESPIRATORY TRACT

吸烟和肺癌之间有明显的联系，但事实并非如此。 仍然不知道为什么有些人，谁是大量暴露于大 高浓度的化学致癌物，不会产生肿瘤，而 其他人会。 这些观察结果提供了间接证据， 涉及易于形成肿瘤的遗传因素。 最近的限制性片段长度多态性（RFLP）研究表明， 13号染色体的正常细胞序列的丢失（在这种情况下， 视网膜母细胞瘤）和11号染色体（在Wilm肿瘤和 膀胱癌）与恶性肿瘤有关。 似乎他 方法通常可用于分析继承的 因此在风险评估中提供信息 治疗肺癌 肿瘤和正常组织形成高分子量DNA 已从30多名癌症患者身上采集样本， 限制性内切酶消化和Southern分析。 初始实验 已经集中在检查基因位于短臂的 染色体11;在肿瘤发生过程中等位基因片段的丢失已经被 在15%的样本中检测到细胞Harvey ras基因座，在13%的 胰岛素位点的样本。 这些实验检查了20多个 这些DNA样本在整个人类基因组中的其他遗传位点 都是教区牧师

There is a clear association between smoking and lung cancer, but it is still not known why some individuals, who are heavily exposed to large concentrations of chemical carcinogens, do not develop tumors, whereas others do. These observations provide circumstantial evidence for the involvement of a genetic factor that predisposes for tumor formation. Recent restriction fragment length polymorphism (RFLP) studies have shown that the loss of normal cellular sequences from chromosome 13 (in the case of retinoblastoma) and chromosome 11 (in the cases of Wilm's tumor and bladder cancer) have been associated with malignancy. It appears that his method might be generally applied to the analysis of inherited susceptibility to cancer and therefore be informative in risk assessment for lung cancer. Tumor and normal tissue form high molecular weight DNA samples have been collected from more than 30 cancer patients for restriction enzyme digestion and Southern analysis. Initial experiments have centered on examination of genes located on the short-arm of chromosome 11; loss of allelic fragments during tumorigenesis have been detected in 15% of samples at the cellular Harvey ras locus and in 13% of samples at the insulin locus. Experiments that examine more than 20 additional genetic loci throughout the human genome for these DNA samples are in parogress.