CLINICAL IMPACT OF MYC FAMILY GENES IN LUNG CANCER

MYC家族(c-myc、N-myc、L-myc)在肿瘤中的扩增研究 小细胞肺癌患者的细胞系和肿瘤标本 与其临床病程相关。 对44个肿瘤细胞系的A.Myc家族DNA扩增进行了研究 由NCI-Naval治疗的小细胞肺癌患者建立 内科肿瘤科。Myc家族的dna扩增更为常见。 在化疗患者建立的细胞系中(11/25) 来自以前未经治疗的患者(2/19)。那些细胞系有 C-myc DNA扩增存活时间显著缩短(中位数33周) 与未扩增c-myc的患者相比(中位数 53周)。在接受治疗的患者中没有观察到存活率的差异。 对N-myc和L-myc进行扩增。 B.28例小细胞肺癌患者的肿瘤标本 学习。从未经治疗的患者身上获得的9份肿瘤标本中没有一份 对Myc家族中的任何一个进行了DNA扩增。19个肿瘤中的4个 从化疗患者身上获得的样本进行了DNA扩增 Myc家族中3例为N-myc，1例为L-myc。 C.将c-myc基因导入小细胞肺癌细胞系 它不表达可检测到的c-myc mrna和转基因克隆。 C-myc基因的表达生长更快，克隆效率更高， 一种改变的大细胞形态。 Myc家族对于理解小细胞肺的生物学很重要。 癌症。基因的DNA扩增与治疗和 C-myc与患者较短的生存期和更大的毒力有关 体外培养小细胞肺癌的表型。

Myc family (c-myc, N-myc, L-myc) DNA amplification was studied in tumor cell lines and tumor specimens from small cell lung cancer patients and correlated with their clinical course. A. Myc family DNA amplification was studied in 44 tumor cell lines established from small cell lung cancer patients treated at the NCI-Navy Medical Oncology Branch. The myc family DNA amplification was more common in cell lines established from chemotherapy treated patients (11/25) than from previously untreated patients (2/19). Patients whose cell lines had c-myc DNA amplification lived significantly shorter (median 33 weeks) compared to patients whose cell lines were not amplified for c-myc (median 53 weeks). No differences in survival was observed in treated patients whose cell lines were amplified for N-myc or L-myc. B. Twenty-eight tumor specimens from small cell lung cancer patients were studied. None of the 9 tumor specimens obtained from untreated patients had DNA amplification of any of myc family. Four of nineteen tumor specimens obtained from chemotherapy treated patients had DNA amplification of the myc family (3 N-myc, 1 L-myc). C. The c-myc gene was transfected into a small cell lung cancer cell line that does not express detectable c-myc mRNA and the transfected clones expressing c-myc mRNA grew faster, had a higher cloning efficiency, and had an altered large cell morphology. The myc family is important in understanding the biology of small cell lung cancer. DNA amplification of the genes is associated with treatment and c-myc is associated with shorter survival in patients and a more virulent phenotype in small cell lung cancer in vitro.