CLINICAL LUNG CANCER RESEARCH PROJECT

人类肺部疾病治疗方法的研究进展 到目前为止，癌症一直令人失望。肺肿瘤的分类 关于它们的新陈代谢潜力对 建立一种系统的抗肺活性物质鉴定方法 肿瘤制剂。因此，我们发起这一项目是为了 制定相关的、可行的人类肺分类方案 肿瘤，以及有效的新肺癌的鉴定 治疗方式。该项目将结合临床方面的 人类肺癌与基础科学和应用科学技术 目前正在努力提供一个全面的计划 肺肿瘤的特征基于其形态， 超微结构、生化、代谢和分子遗传学 特征以及建立体外和体内技术 研究抗原发人类肺癌的潜在抗肿瘤药物。 我们实验室的初步结果证明了这一可行性和 本项目的相关性在于：(1)在 新鲜人前列腺素和4-异丙醇类代谢的研究 肺肿瘤标本，(2)建立和繁殖原代的能力 将为体外药物提供材料的肺肿瘤细胞培养 筛选，建立长期肿瘤细胞系，并进行比较 生物化学研究，(3)人肺肿瘤在 通过一种新的支气管内植入方法移植到裸鼠的肺部，该方法将 允许改进体内繁殖和药物筛选研究。一个 该项目的最终目标是为个性化提供一个范例 肺癌的特征和治疗是对现有技术的改进 接近了。

The development of therapeutic modalities for the treatment of human lung cancer has, to date, been disappointing. Classification of lung tumors with regard to their metabolic potential is particularly important for developing a systematic approach for the identification of active antilung tumor agents. We have, therefore, initiated this project in order to develop a relevant and feasible plan for classification of human lung tumors, as well as for identification of effective new lung cancer treatment modalities. This project will interface the clinical aspects of human lung cancer with basic science and applied science technologies which are currently available in an effort to provide a comprehensive program for characterization of lung tumors based on their morphological, ultrastructural, biochemical, metabolic, and molecular genetic characteristics as well as establish in vitro and in vivo techniques for investigating potential antitumor drugs against primary human lung tumors. The initial results from our laboratories document the feasibility and relevance of this project by demonstrating: (1) considerable variation in prostanoid as well as 4-ipomeanol metabolism among individual fresh human lung tumor specimens, (2) the ability to establish and propagate primary lung tumor cell cultures which will provide material for in vitro drug screening, establishing long term tumor cell lines, and for comparative biochemical studies, (3) the ability to propagate human lung tumors in the lungs of nude mice by a novel intrabronchial implantation method which will allow for improved in vivo propagation and drug screening studies. An ultimate goal of this project is to provide a paradigm for individualized lung cancer characterization and treatment as an improvement over current approaches.