MILK BOMBESIN

牛乳中的蛙皮素(MB)成分已被广泛应用 从获得纯多肽的角度进行了研究 致力于表征牛奶提取物的生物学特性。 提纯工作包括对一种酸性提取物的几次处理 牛奶的成分包括：差速增溶、硫酸铵 沉淀、凝胶过滤、离子交换介质层析以及 采用多种色谱柱和条件进行反相高效液相色谱分析。平滑 肌肉收缩能力研究表明，MB的一个成分 可能具有与两栖动物相似的生物学特性 蛙乙素。然而，在牛乳提取物中有多种形式的MB， 其中一些似乎并不具有生物活性。此外， 有生物活性的牛奶提取物多肽不会 与用于识别MB的抗血清发生交叉反应； 抗血清识别Gly-Asp-Leu-Trp-(残基5-8)肽序列 蛙乙素)。目前仍在继续努力确定平滑肌肉的特征。 牛奶提取物的收缩曲线，并实现最终的纯化 几种低分子量范围-MBS。

The milk bombesin (MB) composition of bovine milk has been extensively investigated from the points of view of obtaining pure peptides and of atempting to characterized the biological properties of the milk extract. The purification efforts involved several manipulations of an acid extract of milk including: differential solubilization, ammonium sulfate precipitation, gel filtration, chromatography on ion exchange media, and reverse phase HPLC using a variety of columns and conditions. Smooth muscle contractility studies have suggested that one of the MB components of bovine milk may have biological properties similar to those of amphibian bombesin. However, there are multiple forms of MB in bovine milk extracts, and some of these do not appear to be biologically active. Furthermore, there are biologically active milk extract peptides which do not cross-react with the antiserum which is used to recognize MB; that antiserum recognizes the peptide sequence -Gly-Asp-Leu-Trp- (residues 5-8 of bombesin). Efforts are continuing to characterize the smooth muscle contractile profiles of milk extracts and to achieve final purification of several of the low-molecular weight range-MBs.