Nuclear RNA surveillance and its connection to splicing quality control

由于RNA剪接容易出错，精细的质量控制过程确保只有正确剪接的转录本才能离开细胞核。人们早就知道，错误剪接的mRNA转录物会被核RNA监视机制降解，但RNA质量控制机制如何与核RNA监视相连接尚不清楚。这一建议旨在揭示这两个重要过程之间的联系，并将填补我们对剪接质量控制和核RNA监测如何工作的理解的重大空白。该项目还将确定触发剪接失败反应的序列特征，从而有助于改进合成mrna的设计。

Due to the error-prone nature of RNA splicing, elaborate quality control processes ensure that only correctly spliced transcripts can leave the nucleus. It has long been known that incorrectly spliced mRNA transcripts are degraded by the nuclear RNA surveillance machinery, but how the RNA quality control machinery is connected to nuclear RNA surveillance is not known. This proposal aims to uncover the connection between these two important processes and will fill a significant gap in our understanding of how splicing quality control and nuclear RNA surveillance work. The project will also identify sequence features that trigger abortive splicing reactions and will thus help to improve the design of synthetic mRNAs.