Significance of efflux pumps in multidrug resistance and pathogenesis of Acinetobacter spp.

不动杆菌引起的医院感染。这是一个严重的问题，尤其是在烧伤和重症监护病房，因为不动杆菌属的快速增加。它们已经对多种抗生素产生了抗药性。在这个项目中，我们将进行实验，使我们能够了解抗生素多重耐药的原因。我们将专注于广泛的底物特异性外排泵，这些泵积极地输出药物，从而使它们的效果变得更差。我们想知道外排泵活性增加在不动杆菌多药耐药发展中的作用。在临床环境中。我们预计我们将发现这些泵的生理底物以及产生它们的生化途径。这可能会导致发现新的抗菌剂和广谱外排泵的抑制剂。

Hospital-acquired infections caused by Acinetobacter spp. are a serious problem especially in the burns and intensive care units because of the rapid increase in Acinetobacter spp. that have become resistant to multiple antibiotics. In this project, we will perform experiments that will allow us to understand how multi-resistance to antibiotics is caused. We will focus on broad substrate-specificity efflux pumps that actively export the drugs, thus rendering them less effective. We want to know the contribution of increased efflux pump activities in the development of multi-drug resistance in Acinetobacter spp. in the clinical environment. We anticipate that we will discover physiological substrates of these pumps and the biochemical pathways that produce them. This could lead to the discovery of novel antimicrobials and inhibitors of the broad-spectrum efflux pumps.