Unravelling multiple antibiotic resistance in Salmonella enterica

解开肠道沙门氏菌的多重抗生素耐药性

• 批准号: G0501415/1
• 负责人: Laura Piddock
• 金额: $ 39.44万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0501415%2F1
• 关键词: Unravelling; multiple; antibiotic; resistance; Salmonella

We are performing experiments that will allow us to understand how salmonella bacteria become multi-drug (antibiotic) resistant (MDR) after exposure to antibiotics. Every year people are exposed to salmonella and some people get ill. The very old and young are particularly vulnerable to this infection and they may be so sick that they need antibiotics to help them recover. Treatment of antibiotic resistant salmonella can be difficult. One way in which bacteria can resist antibiotic action is to pump the drug out of the cell. This is why some bacteria are always resistant to some drugs. Some MDR salmonella produce even more of these pumps than normal strains, making these MDR strains additionally resistant to antibiotics. Whilst we know the identity of some of these pumps, we do not know what controls when they are produced, or what makes the bacterium produce more. New methods allow us to look at what happens to bacteria when we have removed or ‘knocked out’ the pump. Other methods allow us to both inactivate, and label, the antibiotic resistance. By doing this the DNA sequence, and hence the gene/s involved, are found.Understanding how MDR is caused will allow us to develop screening tests to quickly identify this type of resistance. Inhibitors of the pumps or genes that control their production can also be developed.

我们正在进行的实验将使我们能够了解沙门氏菌如何在暴露于抗生素后成为多药（抗生素）耐药（MDR）。每年人们都接触到沙门氏菌，有些人会生病。非常老的和年轻的人特别容易受到这种感染，他们可能病得很重，需要抗生素来帮助他们恢复。抗生素耐药沙门氏菌的治疗可能很困难。细菌抵抗抗生素作用的一种方式是将药物泵出细胞。这就是为什么有些细菌总是对某些药物有抗药性。一些MDR沙门氏菌比正常菌株产生更多的这些泵，使这些MDR菌株额外耐抗生素。虽然我们知道其中一些泵的身份，但我们不知道是什么控制它们何时产生，或者是什么使细菌产生更多。新的方法使我们能够看到当我们移除或“敲除”泵时细菌会发生什么。其他方法使我们能够检测和标记抗生素耐药性。通过这样做，DNA序列，从而涉及的基因/s，被发现。了解MDR是如何引起的将使我们能够开发筛选测试，以快速识别这种类型的耐药性。还可以开发控制其产生的泵或基因的抑制剂。