FLUOROIMMUNOASSAY APPARATUS

具有衰减时间常数的新型稀土络合荧光探针 长于典型有机材料的本底衰减时间常数 提供了极大提高时间解决敏感度的潜力 荧光测定法。然而，为了与放射免疫分析方法竞争，所需的 敏感度的提高如此之大，以至于超载恢复 荧光检测器是一个主要的问题。过去在这方面的大量工作 这个问题在很大程度上不适用于非常低的速度和水平 这种情况下的要求。因此，最初的努力正在进行 用于确定最佳荧光检测装置， 辅助信号过载限制电路。与之相关的问题 在极低样本浓度下的荧光探针将在 与CDC合作，CDC作为另一种方法对此方法感兴趣 艾滋病研究的工具。最后，对《 将学习励磁、光学滤波和数字信号处理 并进行了优化。

New rare earth chelate fluorescent probes with a decay time constant much longer than the background decay time-constant of typical organic materials offer the potential of a great improvement in sensitivity of time resolved fluorimetry. However, to rival radioimmunoassay methods the required sensitivity improvement is so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.