大鼠前胆囊收缩素、脑啡肽原和酪氨酸羟化酶的个体发育-猫眼课题宝

大鼠前胆囊收缩素、脑啡肽原和酪氨酸羟化酶的个体发育

批准号：

3968608

负责人：

A M DUCHEMIN

金额：

$0.0万

依托单位：

NATIONAL INSTITUTE OF MENTAL HEALTH

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3968608

关键词：

brain metabolism cholecystokinin developmental genetics dopamine gene expression locus coeruleus messenger RNA norepinephrine radioimmunoassay substantia nigra tyrosine 3 monooxygenase

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中文摘要

翻译

CCK、脑啡肽编码基因的表达和酪氨酸羟基酶(TH)在大鼠出生前后的研究用相应的特异的cDNAs探针对脑内的mRNA进行定量。在……里面此外，还使用了特异性放射免疫测定来测量 CCK免疫反应肽(CCK-IR)与脑啡肽多肽。用于酪氨酸发育表达的研究比较了羟基酶基因、mRNA在脑实质中的表达。黑质和蓝斑与去甲肾上腺素、多巴胺和同时测定它们的代谢物。

英文摘要

The expression of the genes coding for cholecystokinin (CCK), enkephalin and tyrosine hydroxylase (TH) was studied pre- and post-natally in the rat brain by using the corresponding specific cDNA probes to quantify mRNA. In addition, specific radioimmunoassays were used to measure CCK-immunoreactive peptides (CCK-IR) and enkephalin immunoreactive peptides. For the study on developmental expression of the tyrosine hydroxylase gene, mRNA levels were measured comparatively in the substantia nigra and the locus coeruleus and the levels of noradrenaline, dopamine and their metabolites were measured at the same time.

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