DETERMINATION OF AMINES AND AMINE METABOLITES IN BIOLOGICAL SAMPLES

生物胺在神经传递、新陈代谢中的重要性 对各种生理过程的控制激发了人们对 高精度、高灵敏度定量方法的研究进展 这些胺及其代谢物存在于脑脊液、血浆和 尿液。 我们已经为分析制定了精确的、操作简单的程序 这些物质中的某些物质基于高效液相色谱分离 灵敏的电化学检测，配合使用内部 准确量化的标准。这种方法的效用是 通过选择性和高效的内源乙酰化大大扩展 允许从生物样品中快速分离的胺。使用一种 电活性酰化试剂进一步将这种方法扩展到 准确检测本身不符合的胺 电化学检测(例如，组胺和苯乙胺)。

The importance of biogenic amines in neurotransmission, metabolism, and in control of various physiological processes has spurred intense interest in the development of precise and sensitive methods for the quantitation of these amines and their metabolites present in cebrospinal fluid, plasma and urine. We have developed precise, operationally simple procedures for the analyses of certain of these substances based on HPLC separation using highly sensitive electrochemical detection, coupled with the use of internal standards for accurate quantitation. The utility of the approach has been extended greatly by selective and efficient acylation of the endogenous amines which permits ready isloation from the biological sample. Use of an electroactive acylating reagent further extends this approach to the accurate detection of amines which, themselves, are not amenable to electrochemical detection (e.g., histamine and phenethylamine).