IMMUNOBIOLOGY OF ALEUTIAN DISEASE

该项目的目标是确定免疫机制和病毒 在阿留申病（AD）的发病机制中具有重要的特征。 单克隆抗体用于研究抗原的差异， ADV的菌株，并在体外和体内表征病毒蛋白。 从组织中清楚地描绘出高毒性犹他州I ADV 培养适应的无毒ADV-G菌株。 这种特异性可能是 通过间接免疫荧光（IFA）证实， Crandell猫肾细胞培养物或针对犹他州I组织的培养物 ADV感染貂 使用各种mAb的免疫沉淀分析 确定了特定的抗原决定簇。 当 免疫沉淀定义的反应性与IFA组织 和体外反应模式，很明显， 体内识别的病毒相关抗原决定簇是蛋白水解的， 病毒结构蛋白的产物。 完整的结构ADV蛋白被 未在体内鉴定。 然而，在细胞中检测到了结构蛋白。 当分析ADV-G或犹他州I ADV感染的CRFK细胞时， 因此，在本发明中， 在体内发生蛋白水解，产生小的ADV相关多肽 但在体外没有显著的发现， 是主要的病毒抗原

The goal of this project is to define immune mechanisms and viral characteristics important in the pathogenesis of Aleutian disease (AD). Monoclonal antibodies were used to study antigenic differences among strains of ADV and to characterize viral proteins in vitro and in vivo. Highly virulent Utah I ADV was clearly delineated from the tissue culture-adapted avirulent ADV-G strain. This specificity could be demonstrated by indirect immunofluorescence (IFA) against infected cultures of Crandell feline kidney cells or against tissues of Utah I ADV-infected mink. Immunoprecipitation analyses utilizing various mAbs identified specific antigenic determinants. When immunoprecipitation-defined reactivities were correlated with IFA tissue and in vitro patterns of reactivity it was apparent that the virus-associated antigenic determinants recognized in vivo were proteolytic products of viral structural proteins. Intact structural ADV proteins were not identified in vivo. However, structural proteins were detected in vitro when ADV-G or Utah I ADV-infected CRFK cells were analyzed. Thus, proteolysis occurred in vivo, resulting in small ADV related polypeptides but was not a significant finding in vitro where ADV structural proteins were the predominant viral antigen.