ETHANOL AND CELLULAR CALCIUM METABOLISM

众所周知，酒精会影响发育中的组织，这一点可以从胎儿 酒精综合征，但这种影响的分子机制不是 知道的 在这项研究中，我们使用沙美元鸡蛋，以调查 乙醇对细胞分裂早期阶段的影响。 视频增强 用显微镜观察乙醇对沙粒有丝分裂的影响 美元鸡蛋 我们的初步研究表明，在存在低 浓度（30-50 mM）的乙醇，直接影响包括 精子尾部运动瘫痪和胚胎受精畸形 信封. 后期影响包括细胞质组织的破坏 和抑制有丝分裂。 数据表明，乙醇可能会影响 细胞通过细胞周期和有丝分裂的能力。 类似 当将微摩尔氯化钙注入到 这些鸡蛋。 对沙元蛋的研究可以提供一个实验性的 模型的研究机制的影响，乙醇对 发展的早期阶段。 AtT-20/D16小鼠前额叶细胞内钙离子浓度的测定 垂体瘤细胞和大鼠肝细胞中的测定， 荧光钙指示剂Quin 2。 细胞内的蛋白质增加了四倍 与5 mM KCl溶液相比，在50 mM KCl Krebs溶液中观察到钙 标准Krebs溶液 在正常Krebs溶液中，乙醇（30 mM）引起 大鼠肝细胞内钙浓度增加10%，但 AtT-20/D16细胞在此条件下无明显变化。 这些观察结果表明，乙醇对细胞钙的影响， 代谢在不同的细胞类型中可能不同。

Alcohol is known to affect developing tissues, evidenced by the fetal alcohol syndrome, but the molecular mechanisms of such effects are not known. In this study we have used sand dollar eggs to investigate the effects of ethanol on early stages of cell division. Video-enhanced microscopy was used to study the effects of ethanol on mitosis of sand dollar eggs. Our preliminary study showed that in the presence of low concentrations (30-50 mM) of ethanol, the immediate effects included paralysis of sperm tail motility and distortion of the embryo fertilization envelope. Later effects included disruption of cytoplasmic organization and inhibition of mitosis. The data suggest that ethanol may affect the ability of a cell to progress through the cell cycle and mitosis. Similar results were obtained when micromolar calcium chloride was injected into these eggs. The studies on sand dollar eggs could provide an experimental model for investigating the mechanism of the effects of ethanol on the early stages of development. The intracellular calcium concentration in clonal AtT-20/D16 mouse anterior pituitary tumor cells and in rat hepatocytes was measured with the fluorescent calcium indicator Quin 2. A fourfold increase in intracellular calcium was observed in 50 mM KC1 Krebs solution compared to 5 mM KC1 normal Krebs solution. In normal Krebs solution, ethanol (30 mM) caused a 10% increase in intracellular calcium concentration in rat hepatocytes, but under these conditions AtT-20/D16 cells showed no significant change. These observations suggest that the effects of ethanol on cellular calcium metabolism may vary in different cell types.