IMMUNOCHEMICAL STUDIES OF ACTINOMYCES VISCOSUS POLYSACCHARIDES

粘性放线菌产生的多糖可能促进黏附 这些微生物将糖蛋白吸附在牙釉质上或暴露在牙釉质中 根面。它们可能抑制或增强凝集素介导的共聚集 口腔细菌之间，并促成了菌斑的大部分。这些 多糖还可以诱导抗体的形成，这种抗体可能会阻碍或 促进龋病发生发展。虽然粘性酵母菌产生 几种多糖的化学精细结构有待进一步研究 已澄清。它们在根面致病机制中的作用评价 龋齿也是缺乏症。因此，这项提议旨在 粘性支原体完整细胞表面的定量研究 荧光标记的凝集素结合分析，产生关于 结合部位的性质、数量和亲和力(Ka)； 2-以纯化的形式分离，细胞壁，相关的细胞壁和 Av株T6、T14V和T14Av的胞外多糖 用化学和红外光谱表征了它们的分子结构。 免疫化学方法； 3-检测分离的多糖作为抗原的能力， 抑制唾液涂层羟基磷灰石的细菌附着 微珠和抑制细菌共聚集； 4-检测大鼠和兔的结合部位特异性抗体 针对分离的粘菌多糖抗原的多克隆抗体； 5-用分离的多糖抗原主动免疫大鼠 确定特定聚合物刺激抑制或 口腔感染时疾病的加强和 6-制备鼠抗分离多糖的单抗 抗原，阐明其结合部位的特异性并评价其作用 特异性抗碳水化合物在改善植入和疾病中的作用 无特定病原体的大鼠。

Polysaccharides produced by Actinomyces viscosus may promote adherence of those microorganisms to glycoproteins adsorbed to enamel or exposed root-surfaces. They may inhibit or enhance lectin mediated coaggregation between oral bacteria, and contribute to the bulk of plaque. These polysaccharides can also induce formation of antibodies that may hinder or promote development of dental caries. Although A. viscosus produces several polysaccharides their chemical fine structure remains to be elucidated. Evaluation of their role in the pathogenesis of root surface caries is also lacking. This proposal therefore aims on the surface of intact A. viscosus cells by quantitative fluorochrome-tagged lectin binding assays which yield information on the nature, number and affinity (Ka) of binding sites; 2- to isolate in purified form, cell wall, cell wall associated and extracellular polysaccharides from the T6, T14V and T14Av strains of A. viscosus and characterize their molecular structure by chemical and immunochemical methods; 3- to examine the ability of isolated polysaccharides to serve as antigen, inhibit attachment of bacteria to saliva coated hydroxylapatite coated beads and inhibit bacterial coaggregation; 4- to examine the antibody combining site specificity of rat and rabbit polyclonal antibody to isolated A. viscosus polysaccharide antigens; 5- to actively immunize rats with isolated polysaccharide antigens to determine the ability of specific polymers to stimulate suppression or enhancement of disease upon oral infection and 6- to prepare monoclonal rat antibodies against isolated polysaccharide antigens, elucidate their combining site specificity and evaluate the role of specific anti-carbohydrate in modifying implantation and disease in specific pathogen-free rats.