MONOCLONAL ANTIBODIES REACTIVE WITH HUMAN MAMMARY AND COLON CARCINOMA CELLS

这些研究涉及单克隆抗体的产生和利用， 用于鉴定和表征人类癌相关性的抗体（MAb） 乳腺和结肠上皮的抗原和分化抗原。 这些单克隆抗体被用来更好地了解细胞生物学， 人类癌症的发病机制，并提供用于 人类癌症管理的几个方面。 其中包括： 检测隐匿性肿瘤细胞;进一步确定 “正常”、发育异常和癌细胞群的分化; 血清抗原测定;原发性和转移性肿瘤的放射学诊断 使用放射性标记的原位癌病变（和潜在的治疗） 单克隆免疫损伤原位（和潜在的治疗）， 放射性标记的单克隆免疫球蛋白和片段。 这些研究 分为四个方面的调查：（一）产生和 确定新肿瘤相关抗原的MAb的表征 （TAG-72）：（II）开发和表征单克隆抗体， 癌胚抗原（CEA）上的表位库， 癌细胞群体中差异表达;（III） 针对与以下相关的蛋白质的单克隆抗体的产生和表征 转移性细胞群，和（IV）定义和表征 乳腺和结肠分化抗原。

These studies involve the generation and utilization of monoclonal antibodies (MAbs) to identify and characterize human carcinoma associated antigens and differentiation antigens of mammary and colonic epithelium. These MAbs are being used to better understand the cell biology and pathogenesis of human carcinomas, and to provide reagents for use in several aspects of the management of human carcinomas. These include: detection of occult tumor cells; further defining the degree of differentiation of "normal", dysplastic, and carcinoma cell populations; serum antigens assays; and radiologicalization of primary and metastatic carcinoma lesions in situ (and potentially therapy) using radiolabeled monoclonal immunolesions in situ (and potentially therapy) using radiolabeled monoclonal immunoglobulins and fragments. These studies are divided into four areas of investigation: (I) The generation and characterization of an MAb that defines a novel tumor associated antigen (TAG-72): (II) The development and characterization of MAbs to a repertoire of epitopes on carcinoembryonic antigen (CEA) which are differentially expressed among carcinoma cell population; (III) The generation and characterization of MAbs to proteins associated with metastatic cell populations, and (IV) The definition and characterization of breast and colon differentiation antigens.