IMMUNOGLOBULIN STRUCTURE & DIVERSITY--CHARACTERIZATION OF CELL MEMBRANE PROTEINS

I.基因进化。 重组DNA文库已经从一个 各种野生老鼠。 这些图书馆已经被筛选， 对应于T细胞受体的β链的探针， 免疫球蛋白κ轻链恒定区、κ可变区 基因家族和重链可变区基因家族。 序列 这些基因的分析来自Mus pahari，最远的亲属小鼠 物种到近亲繁殖的动物，揭示了不同的进化模式， 研究的每一个基因的突变。 因此，选择性和进化性 压力似乎对免疫系统的作用完全不同， 重要的结构基因 二.增长和监管。 由P388D1巨噬细胞样 已经鉴定了一种细胞系，其是许多浆细胞瘤所需的 用于体外生长的细胞系。 目前这一因子正在提纯中 正在进行实验，以评估其在 浆细胞发育或正常生长。

I. Gene evolution. Recombinant DNA libraries have been constructed from a variety of wild mouse species. These libraries have been screended with probes corresponding to the Beta chain of the T cell receptor, the immunoglobulin kappa light chain constant region, a kappa variable region gene family and a heavy chain variable region gene family. Sequence analysis of these genes from Mus pahari, the most distantly related mouse species to inbred animals, reveals different patterns of evolution and mutation for each of the genes studied. Thus, selective and evolutionary pressures appear to be acting quite differently on immunologically important structural genes. II. Growth and regulation. A factor produced by the P388D1 macrophage-like cell line has been identified which is required by a number of plasmacytoma cell lines for growth in vitro. This factor is currently being purified and characterized and experiments are being initiated to assess its role in plasmacytomagenesis or normal growth.