ASBESTOS CARCINOGENESIS/CHROMOSOMAL CHANGES IN HUMAN CELLS

石棉引起的染色体异常已在人类 胸膜间皮细胞和支气管成纤维细胞体外培养。 在一次暴露于铁石棉后，染色体数目显著增加， 与未暴露的相比，在间皮细胞中发现了畸变 对照 培养物中畸变细胞的数量进一步增加 用石棉处理两次，主要畸变类型为双着丝粒 染色体和无着丝粒片段（微小染色体）。 的数量 在所有的间皮细胞中观察到非整倍体细胞增加 培养物的传代次数。 无显著 在接触石棉和对照的间皮瘤之间发现了差异 细胞 另一方面，在石棉暴露的成纤维细胞中， 与未暴露的相比，非整倍体细胞显著升高， 对照

Asbestos-induced chromosomal abnormalities have been studied in human pleural mesothelial cells and in bronchial fibroblast cultures in vitro. After one exposure to amosite, a significant increase in chromosomal aberrations was found in mesothelial cells, as compared to unexposed controls. The number of aberrant cells was further increased in cultures treated twice wth asbestos, the major aberration type being dicentric chromosomes and acentric fragments (minute chromosomes). The number of aneuploid cells was observed to increase in all the mesothelial cell cultures with the passage number of the culture. However, no significant differences were found between the asbestos-exposed and control mesothelial cells. In asbestos-exposed fibroblasts, on the other hand, the number of aneuploid cells was significantly elevated, as compared to unexposed controls.