A STUDY OF PROTEINS WITHIN THE CNS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS

利用双向凝胶电泳，我们继续研究了 中枢神经系统中的蛋白质。 首先，我们已经证明了一个数字， 顶叶皮层中蛋白质的浓度发生了变化， 海马和小脑后，无论是双边病变的 蓝斑或新生儿用6-羟基多巴胺治疗。 二是 已经证明长期给予A型单胺 氧化酶抑制剂氯吉林对大鼠作用三周会产生变化 顶叶皮质中五种蛋白质的相对含量， 海马体中的单一蛋白质。 第三，我们确定了 亚细胞定位的约50%的蛋白质上可见 使用大鼠脑产生的二维凝胶。 第四，我们确定了 大鼠穹窿下器官中的八种不同蛋白质， 通过盐负荷和/或水改变相对周转率 剥夺 第五，使用手术中获得的星形细胞瘤样本， 我们已经表明，在蛋白质模式中存在着普遍的一致性， 在研究的20个样本中。 然而，有明显的数量 以及二维蛋白质模式的质的差异 与正常人类大脑皮层相比 第六，我们确定了 一种蛋白质的明显遗传多态性，该蛋白质存在于 Sprague-Dawley大鼠下丘脑。 最后，我们确定了 可溶性谷氨酸的α和β亚型的位置 草酰乙酸转氨酶在大鼠和人皮质的二维凝胶上的表达。

Using two-dimensional gel electrophoresis we have continued our studies of proteins in the central nervous system. First, we have shown that a number of proteins are altered in concentration in the parietal cortex, hippocampus and cerebellum following either bilateral lesioning of the locus coeruleus or neonatal treatment with 6-hydroxydopamine. Second, we have demonstrated that chronic administration of the type A monoamine oxidase inhibitor clorgyline to rats for three weeks produces a change in the relative amount of five proteins in the parietal cortex but only a single protein in the hippocampus. Third, we have determined the subcellular localization of about 50% of the proteins visible on two-dimension gels generated using rat brain. Fourth, we have identified eight distinct proteins within the subfornical organ of the rat that are altered in relative turnover rate by salt loading and/or water deprivation. Fifth, using samples of astrocytoma obtained during surgery, we have shown that there is a general consistency in the protein pattern among the twenty samples studied. However, there are marked quantitative as well as qualitative differences in the two-dimensional protein patterns of astrocytomas compared to normal human cortex. Sixth, we have identified an apparent genetic polymorphism for a protein which is present in the hypothalamus of Sprague-Dawley rats. Finally, we have identified the location of both the Alpha and Beta subforms of soluble glutamic oxaloacetic transaminase on two-dimension gels of rat and human cortex.