STUDIES OF HUMAN T-HELPER CELL TYPE 1 AND TYPE 2 CYTOKINE RESPONSES

辅助性T细胞已被分为不同的亚群， 它们分泌的细胞因子。 TH0细胞分泌IL 2、IL 4和IFN; 分泌IL 2和IFN;和TH 2细胞分泌IL 4。 易感性 某些传染病已被证明是依赖于 主要类型的TH细胞诱导。 有一些证据表明 HIV-1感染的进展与对TH2的偏好相关 细胞 因此，HIV-1感染的疫苗可能是有利的，如果它们 通过募集TH1而不是TH2细胞诱导抗HIV免疫。 B。 已经显示出在小鼠中诱导TH1细胞。 从B开始。流产罐 也刺激人类B细胞在T-独立的方式，我们已经 研究它作为HIV-1蛋白和肽的可能载体， 疫苗 因此，确定B. 流产病毒对人类T细胞的影响 小的静止的人类T细胞是 在蛋白质和mRNA方面显示表达IFN和IL 2，但不表达IL 4。 这些结果表明，B.流产可以刺激TH1反应， 人T细胞，因此作为人的载体可能是有益的 感染了HIV-1。 由于TH1反应在存在下是有利的， IL-12是单核细胞的产物，我们研究了B。流产可能导致 从人单核细胞释放IL-12。 mRNA的PCR检测结果显示，B.牛种 确实从单核细胞中诱导IL-12，为B提供了第二条途径。 流产以诱导TH1样应答。 细胞因子可能在 IGIV治疗后观察到的不良反应。 这是研究 使用人类T细胞和单核细胞。 IGIV显示诱导TNF α， IL-1 β和IL-6。 这些结果可以解释 患者经常出现发热、头痛和腰痛 收到这个产品。

T helper cells have been divided into different subsets based on the cytokines they secrete. TH0 cells secrete IL2, IL4 and IFN; TH1 cells secrete IL2 and IFN; and TH2 cells secrete IL4. Susceptibility to certain infectious diseases has been shown to be dependent on the predominant type of TH cell induced. There is some evidence that progression of HIV-1 infection is associated with a bias towards TH2 cells. Thus, vaccines for HIV-1 infection may be advantageous if they induce anti-HIV immunity by recruiting TH1 rather than TH2 cells. B. abortus has been shown to induce TH1 cells in mice. Since B. abortus can also stimulate human B cells in a T-independent manner we have been studying it as a possible carrier for HIV-1 proteins and peptides as a vaccine. It was therefore of interest to determine what effect B. abortus would have on human T cells. Small resting human T cells were shown to express IFN and IL2, but not IL4 in terms of protein and mRNA. These results indicate that B. abortus can stimulate TH1 responses from human T cells, and as such may be beneficial as a carrier for persons infected with HIV-1. Since TH1 responses are favored in the presence of IL-12, a monocyte product, we studied whether B. abortus could induce IL-12 release from human monocytes. PCR of mRNA revealed that B. abortus does elicit IL-12 from monocytes, providing a second pathway for B. abortus to induce a TH1-like response. Cytokines may play a role in the adverse effects seen following treatment with IGIV. This was studied using human T cells and monocytes. IGIV was shown to induce TNFa, IL-1beta and IL-6 from human monocytes. These results may explain the fever, headache and low back pain frequently experienced by patients receiving this product.