DEVELOPMENT OF AN IMMUNOLOGICALLY BASED COCKROACH ANTIGEN DETECTION SYSTEM

社会经济状况较低的儿童哮喘发病率高， 被认为与过敏原和抗原的存在有关 德国蟑螂Blatetella germanica，经常在公共场所出没 住房发展。 几种主要过敏原，包括96、56和23 kD分子量的蛋白质已被表征;大多数研究 是用蟑螂全身的提取物做的 调查人员 美国农业部的研究人员对 经各种杀虫剂处理后留在住所内的蟑螂残骸 杀虫剂和清洁剂。 然而，由于数量有限， 过敏血清可从儿科患者，过敏原的基础上 制度不现实。美国农业部提供的蟑螂源材料 整个身体以及从蟑螂身上收集的蟑螂碎片样本， 盖恩斯维尔的美国农业部专门设计和建造的住宅， 花期 LRR制备这些材料的冻干提取物;使用6 LRR可使家兔产生抗蟑螂多种蛋白的IgG抗体 碎片 阿肯色州儿童医院（ACH） 确定蟑螂碎片含有已知的过敏原， 这些过敏原作为抗原被兔抗体检测到。 兔抗体结合活性的定性损失与 在免疫印迹实验中，通过各种方法产生的IgE结合损失 LRR提取物的处理。 为了量化这种损失， 在LRR已经开发了使用兔抗体的ELISA型测定。 蟑螂出没地区治理前后的采样方法 美国农业部正在尝试使用各种杀虫剂和清洁剂 设施，以测试残留的免疫活性， 兔抗体ELISA。 在检测到抗原性变化后， ELISA，将在ACH评价样本的过敏原或IgE活性。 计划包括将ELISA系统转移到美国农业部，如果过敏原 破坏与兔抗体ELISA反应平行。 这将允许 在测量杀虫剂和清洁剂的影响方面的进一步发展 通过破坏潜在的过敏原， 蟑螂;这反过来又会减少潜在的哮喘暴露 为儿童创造环境。

High rates of asthma in children of lower socioeconomic circumstances is thought to be related to the presence of allergens and antigens of the German Cockroach Blatetella germanica, which often infests public housing developments. Several major allergens, including 96, 56 and 23 kD molecular weight proteins have been characterized; most research has been performed with extracts of cockroach whole bodies. Investigators at the USDA were interested in the potential immunological activity of cockroach debris left in dwellings after treatment with various insecticides and cleaning agents. However, due to the limited quantities of allergic serum available from pediatric patients, an allergen based system was not realistic. USDA provided source materials of cockroach whole bodies as well as samples of cockroach debris collected from specially designed and constructed dwellings at the USDA in Gainesville, FL. LRR prepared freeze-dried extracts of these materials; using 6 rabbits, LRR raised IgG antibodies to a variety of proteins in cockroach debris. Collaborators at the Arkansas Children's Hospital (ACH) determined that the cockroach debris contained the known allergens and these allergens were detected as antigens by the rabbit antibodies. Qualitative loss of binding activity of the rabbit antibodies correlated with loss of IgE binding in immunoblotting experiments created by various treatments of the LRR extracts. To attempt to quantitate of this loss, an ELISA type assay has been developed at LRR using the rabbit antibody. Methods of sampling cockroach infested areas before and after treatment with various pesticides and cleaners are being attempted at the USDA facilities, to be tested for residual immunological activity in the rabbit antibody ELISA. Following detection of antigenic changes in the ELISA, samples will be evaluated at ACH for allergenic or IgE activity. Plans include transferring the ELISA system to USDA if the allergen destruction parallels rabbit antibody ELISA response. This will permit further development in measuring the effects of pesticides and cleaners by destroying potential allergens that remain after extermination of the cockroaches; this in turn will reduce potential exposure to asthma creating situations for children.