ADVERSE EFFECTS OF OXIDATIVE STRESS DURING PREGNANCY

氧化损伤与以下几种疾病的病理有关 疾病，但直到最近才有人建议在 不利的生殖条件。许多环境和职业 暴露是已知的促氧化剂(例如，许多溶剂和金属)，以及 它们可能会导致氧化损伤，从而对生殖产生不利影响。这 一项研究项目探索了环境与环境之间的关系 暴露、氧化应激和不利的生殖条件。 我们现在正在进行试点工作，主要有两个目标。首先是 继续分析有关水平的现有数据的小样本 金属和其他工业污染物，氧化损伤的标志， 以及孕妇和母婴配对的生殖结局 居住在不同地区的工业企业附近 保加利亚。今年，我们完成了对金属和金属区域的研究 氮氧化物污染。试点工作的第二个目标是 解决抗氧化剂和抗氧化剂标记物的变异量 由于季节和个体内的变化而导致的氧化剂状态 存储程序。20名未怀孕妇女连续献血 样品；结果表明，样品不能储存在 在不改变谷胱甘肽水平的情况下持续几个小时以上 和脂质过氧化作用显著。个体内部的水平 可变性也已经确立。此外，到目前为止，有11名孕妇 已在预订中登记，并正在观察是否有变化 随着怀孕的进展，生物标记物。一种测量饮食和 已经开发出了转换为保加利亚RDA的方法。

Oxidative damage has been implicated in the pathology of several diseases, but only recently has it been suggested to have a role in adverse reproductive conditions. Many environmental and occupation exposures are know pro-oxidants (e.g., many solvents and metals), and they may adversely affect reproduction by causing oxidative damage. This research project explores the relationship between environmental exposures, oxidative stress, and adverse reproductive conditions. We are now conducting pilot work with two major aims. First is continuing analysis of small samples of existing data concerning levels of metals and other industrial pollutants, markers of oxidative damage, and reproductive outcome in pregnant women and maternal-infant pairs living in the vicinity of industrial enterprises in various areas of Bulgaria. This year we have completed studies of areas with metal and with nitrogen oxides pollution. The second aim of the pilot work is to resolve the amount of variability in the markers of anti-oxidant and oxidant status due to seasonal and intra-individual variation and to storage procedures. Twenty non-pregnant women have given serial blood samples; the results indicated that the samples could not be stored at all for more than a few hours without altering the level of glutathione and lipid peroxides significantly. The level of intra-individual variability has also been established. Also, 11 pregnant women to date have been enrolled at booking and are being observed for changes in biomarkers as pregnancy progresses. An instrument to measure diet and to convert to Bulgarian RDA's has been developed.