CORE--CELLULAR RESOURCES

该核心将执行细胞分离和培养程序 项目2、3和4的调查人员。核心将负责 分离和维持心肌细胞，分离和维持心肌细胞 非洲爪哇的卵母细胞，并进行细胞质注射 由分子生物学核心提供的DNA的mRNA或核注射 (核心B)。项目2和项目3都提出了对人和大鼠钾的研究 在小鼠Ltk细胞中表达的通道；这个核心将假设 发展和维护这些文化的责任在最初的 通过集中这些例程，已在核心B中执行了转基因。 实验室功能、资源(调查人员工作、动物数量和 成本)将得到更有效的利用。

This Core will perform cell isolations and culture procedures for investigators in Projects 2, 3, and 4. The Core will be responsible for isolating and maintaining cardiac myocytes, isolating and maintaining Xenopus laevis oocytes, and carrying out either cytoplasmic injections of mRNA or nuclear injections of DNA provided by the Molecular Biology Core (Core B). Projects 2 and 3 both propose studies of human and rat potassium channels expressed in mouse Ltk cells; this Core will assume the responsibility of growing and maintaining these cultures after the initial transfections have been performed in Core B. By centralizing these routine laboratory functions, resources (investigator effort, animal numbers and costs) will be used more effectively.