PHYTOREMEDIATION OF TOXIC WASTE SITES

我们计划确定植物修复有毒废物场地的能力。 在原型实验中，我们将重点关注极地树木的能力 为三氯乙烯(TCE)解毒。杨树能够在存在的情况下生长 高水平的三氯乙烯，并排出和代谢化合物。TCE 转化为三氯乙醇、三氯乙酸和双极性 其结构将被确定的化合物。我们将确定哪一个 无性系代谢植物吸收的最大比例的三氯乙烯。 新陈代谢的程度将通过引入 已知可代谢三氯乙烯。摄取和代谢的实验室研究 TCE将使用气密玻璃设备，并有独立的腔室 根和冠才能决定100的命运：物质 管理。最好的无性系将在温室尺度上进行研究 实验以确定它们吸收和解毒三氯乙烯的能力。 最后，将与世界银行合作进行实地研究。 西方化学公司在塔科马港的一个地点。这些 实地考察将进行2到4年，工作量很大。 仪器，将决定植物吸收和 使三氯乙烯解毒，并将测量化合物的蒸腾程度。 结合这些研究，我们将与Leah Bauer博士合作， 美国农业部，密歇根州东兰辛，以确定摄入雏鸡的影响 以确定这对这些动物是否有任何影响。 我们还将调查是否可以利用植物的能力 新陈代谢。TCE对氯化产品作为环境生物标志物的影响 地下三氯乙烯污染的程度。来自一系列植物组织 将对地点进行三氯乙烯代谢物分析，并与已知水平的 三氯乙烷污染。 使用植物修复污染符合NIEHS的任务，即 减轻有毒物质对人体健康的不良影响。这 该方法是减少污染的“绿色解决方案”， 预计将得到公众的批准。

We plan to determine the ability of plants to remediate toxic waste sites. In prototype experiments, we will focus on the ability of polar trees to detoxify trichloroethylene (TCE). Poplars are able to grow in the presence of high levels of TCE and to transpire and metabolize the compounds. TCE is converted to trichloroethanol, trichloroacetic acid, and two polar compounds whose structures will be determined. We will determine which clones metabolize the largest proportion of TCE absorbed by the plants. The extent of metabolism will be augmented by introducing genes which are known to metabolize TCE. Laboratory studies of the uptake and metabolism of TCE will use gas-tight glass apparatuses with separate chambers for roots and crowns in order to determine the fate of 100: of the material administered. The best clones will be studied in greenhouse-scale experiments to determine their ability to absorb and detoxify TCE. Finally, field studies will be conducted in collaboration with the Occidental Chemical Corporation at a site at the Port of Tacoma. These field studies will take place for 2 to 4 years, will be heavily instrumented, will determine the ability of the plants to take up and detoxify TCE, and will measure the extent of transpiration of the compound. In conjunction with these studies, we will collaborate with Dr. Leah Bauer, USDA, East Lansing, MI, to determine the effect of the ingestion of chicks to determine whether there is any effect on these animals. We will also investigate whether we can use the ability of plants to metabolize. TCE to chlorinated products as an environmental biomarker of the extent of below-ground TCE contamination. Plant tissue from a range of sites will be analyzed for TCE metabolites and compared to known levels of TCE contamination. The use of plants to remediate pollution meets the mandate of the NIEHS to attenuate adverse effects of toxic materials on human health. This approach serves as a "green solution" to pollution abatement and is expected to meet with public approval.