DEVELOPMENTAL BIOLOGY AND GENETICS OF THE MAIZE LEAF

这些拟议研究的总体目标是了解 器官是在植物部分内设计的， 能力随着发展时间的推移而展现。 我们的系统，玉米 茎段和叶器官发育，现在受益于骨骼 一个涉及10个克隆基因的功能网络，其中至少有8个 是转录因子。 其中一些基因对于 初始化段的特定域。 一个是必要的 保持叶子中同源域基因的“关闭”。 我们假设每一个 同源异型盒基因（knox）在节段的不同区域起作用， 能力的“成熟时间表”。我们的建议集中在轴 我们的基因是否参与了一个长期的 范围信号通路。 目标编号l I类KNOX蛋白如何 在段和叶中的功能？ 我们的战略包括跨越我们的 knox基因敲除在一起以产生最小化的品系。 这些线路将 对免疫定位研究有价值，评估KNOX- 功能表型和用于检测KNOX蛋白、运输。 水稻 将被用作对照基因组。 目标二我们将确定是否 RS 2直接结合RS 1基因。 我们将使用酵母单杂交筛选 和蛋白质-DNA狭缝印迹 我们将制备抗RS 2的抗体 蛋白质，以测试该Myb转录因子是否结合 体内knox DNA。 目标三我们将进一步测试 区域命运获得和体细胞的时间表假说 沿着p-d轴沿着继承。 我们希望Knox功能敲除 表型以加速发育。 四号目标我们将测序 ns基因并制备NS蛋白的抗体。目的 5号。 我们将使用马赛克映射来发现，如果有， 表皮是转换ad-ab极性的“主人”， 并克隆了该基因rld 1。 我们打算利用rld 1基因 进一步剖析启动和维持的机制， 背腹性 六号目标。我们将确认基因的克隆 参与远程信号的接收， 发育阶段变化（gef 1），并将标记，克隆和研究lax midrib 1，其显性等位基因Lxm 1-O是一个长距离加速器， 芽发育 我们正在阐明以下疾病的分子遗传基础 异时性，将进化与 发育生物学

The general objective of these proposed studies is to understand how an organ is designed within the plant segment, and how regional competencies unfold over developmental time. Our system, the maize shoot segment and leaf organ development, now benefits from a skeletal network of function involving 10 cloned genes, at least eight of which are transcription factors. Some of these genes are essential for initializing particular domains of the segment. One is necessary to keep homeodomain genes "off' in the leaf. We hypothesize that each homeobox gene (knox) acts in varying regions of the segment to retard a "maturation schedule" of competence. Our proposal focuses on the axes of the leaf, and on whether or not our genes are involved in a long- range signaling pathway. Aim number l. How do Class I KNOX proteins function in the segment and leaf? Our strategy involves crossing our knox gene knockouts together to make minimized lines. These lines will be valuable for immunolocalization studies, assessing loss-of-KNOX- function phenotypes and for testing KNOX protein, trafficking. Rice will be used as a control genome. Aim number 2. We will determine if RS2 directly binds the rs1 gene. We will use a yeast one-hybrid screen and protein-DNA slot-blots. We will prepare an antibody against RS2 protein in order to test whether this Myb transcription factor binds knox DNA in vivo. Aim number 3. We will test further the maturation schedule hypothesis for regional fate acquisition and somatic inheritance along the p-d axis. We expect Knox function knockout phenotypes to accelerate development. Aim number 4. We will sequence ns genes and prepare an antibody to an NS protein, in collaboration. Aim number 5. We will use mosaic mapping to discover which, if either, epidermis is the "master" in switching ad-ab polarities, and will tag and clone the responsible gene, rld1. We intend to use the rld1 gene to further dissect the mechanism of initiation and maintenance of dorsiventrality. Aim number 6. We will confirm a clone of a gene involved in reception of long-range signals involving shoot developmental phase change (gef1), and will tag, clone and study lax midrib1, whose dominant allele Lxm1-O is a long-range accelerator of shoot development. We are elucidating a molecular genetic basis for heterochrony, the prevailing theory that links evolution with developmental biology.