OPTIMIZE HUMAN NEURONAL CELLS FOR SPINAL CORD TRANSPLANT

描述：(逐字摘自申请者的摘要)神经元置换 治疗是治疗脊髓损伤的一种很有前途的方法。这个 HNT神经元的独特性质使其成为一种潜在的细胞来源 目的。HNT神经元是人类纯净的、有丝分裂后、分化后的神经元 可根据商品无限量生产的细胞 制造实践。在移植实验中，这些神经元存活下来， 成熟，生长神经突起，形成突触，整合和恢复功能。这个 建议的研究是基于对神经元可塑性的评价。 以及它们不同的神经递质表型，对于 最佳脊髓替代神经元的发展。目标是不同的 HNT神经元分化过程参数的研究 选定的表型，筛选特定神经元制剂的活性 脊髓组织块体外培养，并对优化后的HNT神经元进行评价 在活体脊髓损伤的动物模型中。初步证据表明 HNT神经元在科学上是有希望的，在技术上是可行的，而且 商业上可行的替代神经元来源，用于治疗 脊髓损伤和其他中枢神经系统疾病。 建议的商业应用：不可用

DESCRIPTION: (Verbatim from the Applicant's Abstract) Neuronal replacement therapy is a promising approach for the treatment of spinal cord injury. The unique properties of hNT-Neurons make tem a potential source of cells for this purpose. hNT-Neurons are pure, post-mitotic, differentiated human neuronal cells that can be produced in unlimited quantities according to Good Manufacturing Practices. In transplantation experiments, these neurons survive, mature, grow neurites, form synapses, integrate, and restore functioning. The proposed research is bas4ed on an appreciation of the plasticity of the neurons and their diverse neurotransmitter phenotypes, important properties for the development of optimal spinal cord replacement neurons. The aims are to vary the parameters of the differentiation process to produce hNT-Neurons with selected phenotypes, screen the activity of specific neuronal preparations in spinal cord explant cultures in vitro, and evaluate the optimized hNT-Neurons in an animal model of spinal cord injury in vivo. Preliminary evidence suggests that hNT-Neurons are scientifically promising, technically feasible, and commercially viable as a source of replacement neurons for the treatment of spinal cord injury and other CNS disorders. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE