PROTEINS OF NORMAL AND CATARACTOUS LENSES

描述(改编自申请人的摘要)：总体目标 应用的重点是评价氧化应激在 人类老年性白内障的发展。该应用程序将研究 豚鼠核性白内障模型的形成 在变老的人类中形成。豚鼠在体内形成核性白内障 对高压氧的反应。传统技术，如拉曼 光谱分析和双向凝胶电泳法，SDSPAGE和 免疫印迹分析将与更具创新性的方法相结合，如 正如M·M博士开创的晶状体蛋白质二硫键LC分析方法。 卢，一个合作者。这些分析将提供有关 膜结合聚集和可溶性的相对贡献率 晶状体蛋白聚集对核性白内障混浊的影响 高压氧/豚鼠模型。虽然应用程序的元素 例如对基因敲除和转基因动物的研究可能是 为时过早，应用通常是非常强大和重要的新 关于白内障形成的信息是可以预期的。

DESCRIPTION (Adapted from applicant's abstract): The overall objective of the application is the evaluation of the role of oxidative stress in the development of human senile cataract. The application will study formation of nuclear cataract in a guinea pig model for nuclear cataract formation in aging humans. The guinea pig forms a nuclear cataract in response to hyperbaric oxygen. Traditional techniques such as Raman spectroscopy and 2-dimensional gel electrophoresis, SDS PAGE and immunoblot analyses will be combined with more innovative methods such as LC assays for disulfide bonds pioneered for lens proteins by Dr. M. Lou, a collaborator. The analyses will provide information on the relative contribution of membrane-associated aggregation and soluble crystallin aggregation to opacification in nuclear cataract of the hyperbaric oxygen/guinea pig model. While elements of the application such as the studies with knockout and transgenic animals may be premature, the application is generally very strong and important new information on cataract formation can be expected.