Y CHROMOSOME POPULATION GENETICS

Y染色体微卫星位点突变率、人类Y染色体系统发育和人类种群分化日期的估计是本项目的目标。主要从5个亚洲人和11个美洲土著人群样本中抽取的约500个人，正在对9个微卫星位点和5个具有已知祖先状态的非重复位点进行基因分型，这些位点均位于人类Y染色体的非重组、非假常染色体区域。根据收集到的基因型构建单倍型，并计算群体遗传参数（包括杂合度和等位基因重复单位方差），比较群体多样性和单倍型/群体相关性。使用距离（种群方差）和简约（单倍型间距离）方法的系统发育分析将构建进化相关种群和单倍型的网络。连锁不平衡统计数据将计算，以估计微卫星突变率使用群体建模方法，适应自常染色体连锁不平衡作图方法。这些分析将有助于了解亚洲祖先种群向亚洲和美洲的分散和迁移，并将通过综合种群遗传、系统发育和连锁不平衡分析来描述后代种群之间的关系。-人口研究，饮酒模式和原因，基因定位（人类），分子遗传学-人类受试者

The estimation of the mutation rates of Y chromosome microsatellite loci, human Y chromosome phylogeny and human population divergence dates are the goals of this project. Approximately 500 individuals, drawn primarily from 5 Asian and 11 Native American population samples, are being genotyped at 9 microsatellite loci and 5 non-repetitive loci with known ancestral state, all located on the non-recombining, non- pseudoautosomal region of the human Y chromosome. Haplotypes will be constructed from the collected genotypes and population genetic parameters including heterozygosity and allelic repeat unit variance statistics will be calculated to compare population diversities and haplotype/population associations. Phylogenetic analysis using distance (population variance) and parsimony (interhaplotypic distance) methods will construct networks of evolutionarily-related populations and haplotypes. Linkage disequilibrium statistics will be calculated to estimate microsatellite mutation rates using population modeling approaches adapted from autosomal linkage disequilibrium mapping methods. These analyses will contribute to a understanding of the dispersal and migration of ancestral Asian populations into Asia and the Americas and will describe the relationships among descendent populations through the combined population genetic, phylogenetic and linkage disequilibrium analyses performed. - population research, drinking patterns & causes, gene mapping (human), molecular genetics - Human Subjects