RAPID ENZYME AND RECEPTOR ASSAYS USING BIOSOMES

描述(逐字摘自申请人的摘要)：这项研究将 开发用于药物发现的新的分子识别技术。稳定 脂质体聚合物，称为生物小体，充当生物传感器，从蓝色变成 红色是对特定分子识别事件的响应。生物小体可以是 用酶底物或受体配体官能化，以便它们 对特定的受体或酶作出反应。一种典型的酶分析已经被 使用磷脂酶开发的。生物小体也会改变颜色以响应 与受体的相互作用。第二阶段研究将把这些研究扩展到 发展多种酶检测方法。约束性研究将继续进行 目的是发展快速的同源受体结合分析。此外, 将努力通过以下方式大幅提高生物小体分析的灵敏度 将荧光标记引入生物小体聚合物。生态体的颜色 作为对分子识别的回应，过渡将被用于改变 通过颜色猝灭或荧光能量的变化进行荧光 调职。生物小体在高通量方面应该有很多应用 为广泛的治疗领域筛选化合物。 建议的商业应用：不可用

DESCRIPTION (Verbatim from the applicant's abstract): This research will develop a new molecular recognition technology for drug discovery. Stable liposome polymers, called biosomes, act as biosensors that turn from blue to red in response to a specific molecular recognition event. Biosomes can be functionalized with either an enzyme substrate or receptor ligand so that they respond to a specific receptor or enzyme. A prototypic enzyme assay has been developed using phospholipases. Biosomes also change color in response to interactions with receptors. Phase II research will extend these studies to develop a variety of enzyme assays. The binding studies will be continued with the aim of developing rapid homogenous receptor binding assays. In addition, efforts will be made to greatly increase the sensitivity of Biosome assays by incorporating fluorescent labels into the Biosome Polymers. The Biosome color transition, in response to molecular recognition, will be used to alter fluorescence by means of changes in color quenching or fluorescence energy transfer. The Biosomes should have many applications in high-throughput screening of compounds for a wide range of therapeutic areas. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE