CORE--ANIMAL

动物核心的目标是为研究人员提供 计划项目为设计提供科学、技术和资金支持 以及执行他们的研究。集中化状态的可用性 收购和安置费舍尔的艺术设施是必要的 344只大鼠将用于脑缺血再灌流和抗氧化 干预性研究，即肿瘤坏死因子在衰老和缺血中的表达 再灌流对复合体III的心脏损伤，氧化修饰 Rieske铁蛋白、心磷脂状态和游离脂肪酸氧化 硫醇防御机制。此外，还需要该设施来 对病原体进行现场短期和长期干预研究- 自由环境与新型加速模式的发展 衰老。 这个核心的具体目标是：1.协调收购和 费希尔344只大鼠及其组织在计划项目中的分布 参与缺血再灌注研究的研究人员，2.提供 为参与这些研究的Fischer 344只大鼠和其他啮齿动物提供住所 在一个集中的、专门的设施下，定期监测 遵守适用于老年学研究的健康标准 (无病原体环境、体重测量、身体和 组织学检查)，3.为科学设计提供帮助 以及执行涉及特殊饮食和药理的协议 研究衰老动物的治疗方法，以及4.为新的 微型隔离器使用方案项目的调查人员 以及与老年学研究和研究有关的特殊技术 涉及无病原体的动物污渍。 为方案项目的调查人员提供的服务将由 由核心总监(V.Monnier)组成的团队，由2名共同- 董事，即动物科学专家和障碍设施 对NIA啮齿动物的长寿研究(Sell博士，博士)和一位兽医(N. Kleinman，DVM)。50%全时当量的研究助理将承担日常工作 与动物中心的工作人员合作运营该设施 CWRU资源中心。 预计这个设计广泛的动物核心将使我们能够 整合与计划项目相关的新项目，同时 发挥其主要作用。

The objective of the Animal Core is to provide investigators of the Program Project scientific, technical and financial support for the design and execution of their studies. The availability of a centralized state of the art facility is needed for the acquisition and housing of the Fischer 344 rats that will be utilized in the ischemia-reperfusion and antioxidant intervention studies, i.e. TNF expression in the aging and ischemia- reperfused heart damage to complex III, oxidative modification of the Rieske iron protein, cardiolipin status and free fatty acid oxidation and the thiol defense mechanism. In addition, the facility will be needed to conduct on-site short and longer term intervention studies in a pathogen- free environment and the development of a novel model of accelerated aging. The specific aims of this core are 1. To coordinate the acquisition and distribution of Fischer 344 rats and their tissues among Program Project investigators involved in ischemia-reperfusion studies, 2. To provide housing for Fischer 344 rats and other rodents involved in these studies under a centralized, specialized facility routinely monitored for compliance with standards of health applicable to gerontological studies (pathogen free environment, body weight measurement, physical and histological examination), 3. To provide assistance with scientific design and execution of protocols involving special diets and pharmacological treatments to study aging animals, and 4. To provide training for new investigators of the Program Project in the utilization of micro-isolators and special techniques pertinent to gerontological studies and studies involving pathogen-free animal stains. Services to the Investigators of the Program Project will be provided by a team consisting of the Core director (V. Monnier) assisted by 2 Co- directors, i.e. an expert in Animal Sciences and Barrier Facility for longevity studies with NIA rodents (DR. Sell, PhD) and a Veterinarian (N. Kleinman, DVM). A 50% FTE Research Assistant will assume the day-to-day operation of the facility in collaboration with the staff of the Animal Resources Center at CWRU. It is expected that this broadly designed Animal Core will allow us to integrate new projects of relevance to the Program Project while fulfilling its primary role.