LYME DISEASE--PATHOGENESIS AND PROTECTION

这个富有成效的项目寻求继续学习的支持 研究莱姆病的发病机制和保护性免疫 细胞、分子、遗传和免疫学方法的数量 一个很有特色的小鼠模型。该计划由五个互动项目组成 项目和三个支持核心： 项目1将调查宿主对肩部硬蜱的免疫力和 评估诱导的壁虱免疫对感染性的影响， 蜱传疏螺旋体的传播和表面抗原表达 勃格多费里。 项目2通过体外、体外和原位研究，将提供一种 B。吞噬细胞功能完整性的全貌。 感染伯氏杆菌的宿主。 项目3将研究不同的伯氏杆菌基因的调控。 在扁虱载体、培养物和哺乳动物宿主中表达。 项目4将确定伯氏假单胞菌是否具有外部变量 不结合保护性抗体从而抵抗的表面蛋白 被宿主消除，优先在受感染的小鼠体内存活 人类，从而促成了莱姆病的发病机制。 项目5正在调查莱姆疏螺旋体病的发病机制。 老鼠模型。该项目在以下背景下获得财政支持 这一POL，但与POL的努力密不可分。因此，它是 作为项目包括在内(不要求额外资金)，以提供 评论者对我们的节目有一个全球的视角. 核心A将支持本计划的管理。 核心B将提供标准化的媒体、动物接种、免疫接种、 组织收集、组织学和专业病理学服务，以 支持本计划的项目。

This highly productive Program seeks support to continue studies investigating Lyme disease pathogenesis and protective immunity, using a number of cellular, molecular, genetic, and immunological approaches with a well-characterized mouse model. The Program consist of five interactive projects and three supporting cores: Project #1 will investigate host immunity to Ixodes scapularis ticks and evaluate the effect of induced tick immunity upon infectivity, transmission and surface antigen expression of tick-borne Borrelia burgdorferi. Project #2, through studies in vitro, ex vivo and in situ, will provide a comprehensive picture of the functional integrity of phagocytes in the B. burgdorferi-infected host. Project #3 will study the regulation of differential B. burgdorferi gene expression in the tick vector, culture and the mammalian host. Project #4 will determine whether B. burgdorferi, with variable outer surface proteins that do not bind protective antibody and therefore resist elimination by the host, preferentially survive within infected mice and humans, thereby contributing to the pathogenesis of Lyme disease. Project #5 is investigating the pathogenesis of Lyme borreliosis in a mouse model. This project is financially supported outside the context of this POl, but is integrally related to the POl effort. It is therefore included as a project (without request for additional funding) to provide the reviewers a global perspective of our program. Core A will support the administration of this Program. Core B will provide standardized media, animal inoculation, immunization, tissue collection, histology and professional pathology services to support projects of this Program.