CORE--PROTEIN AND MOLECULAR BIOLOGY FACILITY

这个项目的核心是蛋白质和分子生物学 项目将在Rheumatic现有设施内运营 疾病司支持计划的研究活动 调查人员。这些研究人员提出的研究计划有 试剂制备的通用要求，分配 核酸的供应、合成和分析，纯化的表达 蛋白质与体外基因转移用重组DNA的制备 转基因小鼠的实验和生成。核心将由一个 主任、研究科学家和研究技术员。《核心》 设施将包括DNA合成器和自动DNA测序仪， 长期的组织培养设施，以及用于准备的实验室 重组DNA和蛋白质的纯化。这些计划和 核心提供的服务将包括： 1.DNA合成和测序 2.杂交瘤蛋白生产 3.重组蛋白生产 核心与主要调查员之间的密切合作 将对个别项目进行维护，以优化其 通过利用核心服务来研究工作效率。就这样， 将避免浪费的冗余，并将研究试剂 以更低的成本提供。这将允许不常见的集中化 采用的技术，导致更好的质量控制，更快 吞吐量。

The Protein and Molecular and Molecular Biology Core of this Program Project will operate within the existing facilities of the Rheumatic Diseases Division to support the research activities of Program investigators. The research programs proposed by these investigators have common requirements for preparation for reagents, distribution of supplies, synthesis and analysis of nucleic acids, expression of purified proteins and generation of recombinant DNA for in vitro gene transfer experiments and generation of transgenic mice. The Core will consist of a Director, a Research Scientist, and Research Technician. The Core facilities will include a DNA Synthesizer, and Automated DNA Sequencer, long-term tissue culture facilities, and a laboratory for the preparation and purification of recombinant DNA and proteins. The programs and services provided by the Core will be: 1. DNA Synthesis and Sequencing 2. Hybridoma Protein Production 3. Recombinant Protein Production Close collaboration between the Core and the principal investigators for the individual projects will be maintained in order to optimize their research productivity through utilization of Core services. In this way, wasteful redundancy will be avoided, and research reagents will be provided at reduced costs. This will allow centralization of uncommonly used techniques, resulting in better quality control, more rapid throughput.