Core--Immunohistopathology

描述(摘自研究人员摘要)：免疫组织病理学核心C将提供协调和有效的设施，允许对组织样本进行可重复和可预测的组织化学处理，随后将通过自动或半自动图像分析技术进行分析。联合首席调查员将监督一个在定量组织形态计量学、细胞计数、免疫组织病理学、原位杂交、凝胶分析和放射自显影方面拥有既定记录的团队。这种方法可能会产生准确性和可重复性的结果。联合首席调查员和工作人员将对设施进行监督，包括安排时间表、培训项目人员和监督图像分析设施的使用。本提案将使用的增强型分析系统是独一无二的，因为它取代了以前用于这一目的的各种不太敏感、繁琐的技术。该设施的技术人员都将精通拟议成像中心的硬件和软件的使用，因为它适用于免疫病理学、密度测定和荧光技术。所需的额外技术员将为项目2-4提供支助。

DESCRIPTION (adapted from investigator's abstract): The Immunohistopathology Core C will provide coordinated and efficient facilities to allow reproducible and predictable histochemical processing of tissue samples that will be subsequently analyzed by automated or semiautomated image analysis techniques. The co-principal investigators will supervise a team that has an established record in quantitative tissue morphometrics, cell counting, immunohistopathology, in situ hybridization, gel analysis, and autoradiography. Accuracy and reproducible outcomes are likely to result from the approach. The co-principal investigators and staff will provide oversight for the facility including scheduling, training of project personnel, and supervising use of the image analysis facilities. The enhanced analysis system that will be used in this proposal is unique in that it replaces a variety of less sensitive, cumbersome technologies that have previously been used for this purpose. Technical personnel in the facility will all be well versed in the employment of the hardware and software in the proposed imaging center, as it applies to immunopathology, densitometry, and fluorescent techniques. The additional technician requested will provide support to Projects 2-4.