Risk Factors for Cell Transformation and the Role of Apoptosis

维持正常的机体内环境平衡 通过仔细平衡细胞增殖和细胞死亡 流程。癌细胞，在经历快速分裂和 扩张，也可能经历细胞死亡。重要的是要 了解癌细胞如何死亡或失去对 细胞死亡过程。我们已经证明，细胞的敏感性在 肿瘤过程的不同阶段经历细胞凋亡 有所不同。在单独的研究中，我们正在探索细胞凋亡的途径。 以应对氧化损伤。在跟踪研究中的作用 在P53的这个过程中，我们发现细胞凋亡也出现了 需要P85激酶。此外，我们还发现RB 蛋白质可能是细胞决定是否要 受伤后逮捕或自杀。此外，我们还 我一直在研究EIA结合的p300蛋白作为一种 多种细胞反应的积分器。我们发现了p300 在细胞应激条件下能与c-fos蛋白偶联 这会导致细胞凋亡。C-fos的上升已被证明是 低血清诱导金黄地鼠成纤维细胞凋亡的关键事件。 我们认为c-fos与p300的结合对于 细胞决定经历细胞死亡。此外，我们正在 开始研究对各种疾病的早期基因反应 引发氧自由基的物质。我们希望用cDNAs 微阵列技术建立分子标记的研究 细胞对这类药物的反应。

Normal organismal homeostasis is maintained through a careful balance of cell proliferation and cell death processes. Cancer cells, while rapidly undergoing division and expansion, may also undergo cell death. It is important to understand how cancer cells die or lose their susceptibility to the cell death process. We have shown that the susceptibility of cells at various stages of the neoplastic process to undergo apoptosis differs. In separate studies, we are pursuing the apoptotic pathway in response to oxidative damage. In studies following the role of p53 in this process, we have found that apoptosis also appears to require the p85 kinase. In addition we have found that the Rb protein may be a key player in the cellular decision whether to arrest or commit suicide following injury. In addition, we have also been examining the role of the EIA binding, p300 protein, as an integrator of multiple cellular responses. We have found that p300 can couple with the c-fos protein under conditions of cellular stress that result in apoptosis. The elevation of c-fos has been shown to be a key event in low serum induced apoptosis of hamster fibroblasts. We propose that the binding of c-fos to p300 is critical for the cellular decision to undergo cell death. In addition, we are beginning studies to examine the early gene response to a variety of agents that induce oxygen free radicals. We hope to use cDNA microarray technology to establish a molecular signature of the cellular response to this class of agents.