CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODIES

组织培养和单克隆抗体（TCMA）实验室的目标 保持这一核心融资的原始提案所述的内容： 1. 为了维持一个具有成本效益的集中设施， 并且单克隆抗体/杂交瘤相关的活性可以通过 对于劳伦斯医学中心的研究人员来说， MRDRG。 2. 协助研究者进行原代细胞的生长和维持 用于多种分子和细胞生物学的培养物和细胞系 问题研究 3. 进行单克隆抗体的生成和表征 并维持杂交瘤细胞系， 研究项目需要持续供应的研究人员 抗体的 4. 提供荧光激活流式细胞术功能， 特定细胞表型的分析和各种 实验性治疗

The goals for the Tissue Culture and Monoclonal Antibody (TCMA) Laboratory remain as outlined in the original proposal for this Core facility: 1. To maintain a cost-effective centralized facility where cell culture and monoclonal antibody/hybridoma-related activities can be performed by and for investigators at the lawrence and the Medical Center settings of the MRDDRG. 2. To assist investigators in the growth and maintenance of primary cell cultures and cell lines used in a variety of molecular and cellular studies. 3. To carry out generation and characterization of monoclonal antibodies for MRDDRC investigators and maintain hybridoma cell lines for the investigators whose research programs require a consistent supply of antibodies. 4. To provide fluorescence activated flow cytometry capabilities for analysis of specific cell phenotypes and the effects of various experimental treatments.