DEPARTMENTAL MASS SPECTROMETRY FACILITY

本申请书寻求购买两台质谱仪的资金 组成一个部门设施：(1)一个芬尼根LCQ桌面MS系统 具有电喷和化学电离能力，关联梯度 LC泵系统，正负离子检测，质量/电荷范围 50-2000道尔顿；和(2)一台芬尼根SSQ 7000 MS/DS质量垫 具有电子碰撞、化学电离和快原子的光谱仪 轰击能力，正负离子探测，以及 质量/电荷范围可达2500道尔顿。这两个系统都包括功能强大的 用于仪器控制和数据的多功能捆绑式计算机系统 操纵。 拟议的设备旨在取代过时的单一设备 部门文书，不能再使其达到适当水平 关于表演。获取可靠的质谱学数据至关重要 该部门的所有研究小组的活动 从事化学和生化合成。目前这一点的缺乏 能力是各种NIH进步的主要障碍- 资助的项目以及其他机构支持的项目。这个 所要求的仪器是现代的“主力”机器，具有重要的 以前部门用户无法使用的功能。这个 仪器的设计可以容纳两种主要类型的样品。 由我们的研究小组产生：标准固体和液体小分子 样品，包括有机、无机和有机金属化合物，将 由SSQ-7000提供服务，而生物分子、生物聚合物和 将对其他大小可极化结构进行常规分析 使用LCQ电喷雾仪。考虑到不同的活动性质 该部门关于这两种分子的合成努力， 这两款仪器的用户基数将会很大。

This application seeks funds for the purchase of two mass spectrometers to comprise a departmental facility: (1) a Finnegan LCQ Benchtop MS System with electrospray and chemical ionization capability, associated gradient LC pump system, positive- and negative-ion detection, mass/charge range of 50-2000 daltons; and (2) a Finnegan MAT Model SSQ 7000 MS/DS mass spectrometer with electron impact, chemical ionization, and fast atom bombardment capability, positive- and negative-ion detection, and mass/charge range to 2500 daltons. Both systems include powerful and versatile bundled computer systems for instrument control and data manipulation. The proposed equipment is designed to replace the single outdated departmental instrument, which can no longer be brought to adequate levels of performance. The acquisition of reliable mass spectral data is vital to the activities of all of the research groups in the department that engage in chemical and biochemical synthesis. The current lack of this capability is a major impediment to the progress of a variety of NIH- funded programs as well as projects supported by other agencies. The requested instruments are modern "workhorse" machines that have important capabilities previously unavailable to departmental users. The instruments are designed to accomodate the two major types of samples generated by our research groups: standard solid and liquid small-molecule samples, including organic, inorganic, and organometallic compounds, will be served by the SSQ-7000, whereas biological molecules, biopolymers and other large and small polarizable structures will be routinely analyzed with the LCQ electrospray instrumentation. Given the vary active nature of the department's synthetic efforts concerning both types of molecules, the user base for the two instruments will be large.