PURCHASE OF AN X RAY GENERATOR AND IMAGE PLATE DETECTOR

需要资金购买Rigaku RU-300型X射线发生器，R轴 IV图像板系统和X-STREAM低温晶体冷冻装置， 和硅图计算机工作站，以方便X射线 高分子的结晶学研究。现有的仪器设备 促进了结晶学研究的重大扩展 威斯塔尔研究所。这种结构研究活动的增加已经 达到了缺乏足够的工具资源的地步 在很大程度上阻碍了在研究重要的 大分子。所请求的指令插入将提供资源 满足研究所不断增长的晶体需求所必需的 给由8个实验室组成的主要用户群体，这些实验室拥有大量NIH 给予支持。这些实验室(Berger，Burnett，Halazonetis，Herlyn， 利伯曼、Marmorstein Rauscher和Speicher)正在研究该结构 以及核转录中大分子的功能 因子和细胞骨架蛋白对整个病毒的影响。 X射线仪器将安装在X射线衍射仪中 维斯塔尔研究所的实验室。仪器设备将联合 由P.I.(Ronen Marmorstein博士)和Co-P.I.(Roger M. Burnett)。他们将负责仪器安装，培训， 以及日常运作。内部咨询委员会包括 所有主要用户，以及科学行政副主任， 将监督公平获取、运营和管理 乐器。维斯塔尔研究所致力于住房、维护和 在所请求的仪器的有效使用寿命内配备人员，由 一封来自署长的随附信件。

Funds are requested to purchase a Rigaku RU-300 X-ray generator, R-AXIS IV image plate system and X-stream cryogenic crystal freezing apparatus, and a Silicon Graphics computer work station to facilitate X-ray crystallographic studies of macromolecules. The existing instrumentation has facilitated a major expansion of crystallographic investigations at the Wistar Institute. This increased activity in structural studies has reached the point where the lack of sufficient instrumentation resources is significantly impeding progress in studying the structures of important macromolecules. The requested instrumentation will provide the resources required to meet the escalating crystallographic demands of the Institute to a major user group composed of 8 laboratories with substantial NIH grant support. These laboratories (Berger, Burnett, Halazonetis, Herlyn, Lieberman, Marmorstein Rauscher, and Speicher) are studying the structure and function of a wide range of macromolecules from nuclear transcription factors and cytoskeletal proteins to whole viruses. The X-ray instrumentation will be housed in the X-ray diffraction laboratory at The Wistar Institute. The instrumentation will be jointly managed by the P.I. (Dr. Ronen Marmorstein) and the Co-P.I. (Dr. Roger M. Burnett). They will be responsible for instrument installation, training, and the day-to-day operational. An internal advisory committee comprised of all major users, and the Associate Director of Science Administration, will oversee equitable access, operation and administration of the instrument. The Wistar Institute is committed to housing, maintaining and staffing the requested instrument for its useful lifetime as verified by an accompanying letter from the Director.