LCQ MASS SPECTROMETER SYSTEM

这项提案寻求资金购买一台LCQ TM MS n离子陷阱质量 生物医学光谱仪系统(加利福尼亚州圣何塞的Finnigan公司) 质谱学核心实验室位于 纽约医学院药理学，瓦尔哈拉，纽约州。新的 仪器将用于电喷雾电离(ESI/MS)模式 并在电喷雾串联质谱学模式(ESI/MS/MS)中 阐明新的复杂生物分子的结构。新仪器 将主要用于花生四烯酸(AA)代谢物的分析 用我们现有的GC/MS仪器(HP 5989A， 质量引擎)。这些包括含有酯化的磷脂。 羟基-AA(HETE)，环氧-AA的谷胱甘肽偶联物(EET)， 含有HETE、20-HETE的葡萄糖醛酸苷和核酸的二甘油酯 环氧烯丙基醇加合物。这些完好无损的化合物不是 适用于GC/MS分析，主要是因为高极性和 热不稳定。我们还计划开发分析方法， 定量地同时，几种AA代谢物合而为一 注射。这将使我们能够更全面地了解 细胞色素P450和脂氧合酶对AA的代谢。在一个项目中， 我们建议研究谷胱甘肽与 过氧亚硝酸盐，需要分析硝基和亚硝基谷胱甘肽。 我们得到的初步数据表明，这些化合物很好 经ESI/MS/MS分析，建议的分析重点是生成 血管系统、肾脏和肿瘤中的新化合物 细胞和新的数据预计将提供重大进展 血管生物学、肾功能和癌症领域。少校 用户组由美国国立卫生研究院资助的6名科学家组成(3个优点 获奖者和2名计划项目赠款调查人员)有项目 介绍血管药理学和生物化学的各个领域 系统、肾脏和癌症。长期目标是提供 对长期资助项目和新项目的ESI/MS(/MS)分析 项目。新仪器将在以下方面取得重大进展 了解二十烷类化合物和谷胱甘肽在健康和健康中的作用 疾病。

This proposal seeks funds to purchase an LCQ TM MS n ion trap mass spectrometer system (Finnigan Corp., San Jose, CA) for the Biomedical Mass Spectrometry Core Laboratory located in the Department of Pharmacology of New York Medical College, Valhalla, NY. The new instrument will be utilized in the electrospray ionization (ESI/MS) mode and in the electrospray tandem mass spectrometry mode (ESI/MS/MS) to elucidate structures of new complex biomolecules. The new instrument will be used primarily for analyses of arachidonic acid (AA) metabolites which cannot be analyzed using our existing GC/MS instrument (HP 5989A, Mass Engine). These include phospholipids containing esterified hydroxy-AA (HETE), glutathione conjugates of epoxy-AA (EET), diglycerides containing HETE, glucuronide of 20-HETE, and nucleic acid epoxyallylic alcohol adducts. These compounds in an intact form are not suitable for GC/MS analyses primarily because of high polarity and thermal instability. We also plan to develop methods to analyze, quantitatively and simultaneously, several AA metabolites in one injection. This will allow for a more comprehensive look into metabolism of AA by cytochrome P450 and lipoxygenases. In one project, we propose to study the chemistry of the reaction of glutathione with peroxynitrite which requires analysis of nitro- and nitrosoglutathione. We obtained preliminary data which suggest that these compounds are well analyzed by ESI/MS/MS. The proposed analyses focus on the generation of the new compounds in the vascular system, in the kidney, and in tumor cells and the new data is expected to provide significant advances in the areas of vascular biology, renal function and cancer. The major user group consists of six scientists funded by the NIH (3 MERIT awardees and 2 Program Project Grant Investigators) who have projects addressing various areas of pharmacology and biochemistry of vascular system, kidney and cancer. The long term goals are to provide ESI/MS(/MS) analyses for long-term funded projects as well as for new projects. The new instrumentation will lead to major advances in understanding the role of eicosanoids, and glutathione in health and disease.