INTEGRATED CELLULAR FLUORESCENCE ANALYSIS FACILITY

这项提议的目的是获得设备，以创建一个 医用集成细胞荧光分析设备 南卡罗来纳大学(MUSC)。建议的关键组件 设施包括一个荧光激光成像板阅读器(FLIPR)和一个 FACSCalibur分析流式细胞仪。这项设施是由 三所学院(医学、牙科医学、 和药学)和学院内的6个系 医学(医学、微生物学、药理学、病理学、生理学、 和实验肿瘤学)。具体目标如下： 1.获得荧光激光成像板阅读器(FLIPR)和 FACSCalibur流式细胞仪检测MUSC细胞荧光 分析设施。2.利用这一设施提高生产率 NIH资助的研究人员，并提高研究效率和 MUSC研究和受训人员的竞争力。3.使用本设施 促进用户之间的跨学科工作。 该设施满足了对灵活和高产量的广泛需求 支持许多不同学科的研究的荧光分析。 详细的组织计划，实质性的机构承诺 支持，以及长期的维护计划，确保持续 设施的生存能力。集成蜂窝的可用性 荧光分析设施将为 细胞荧光检测新用途的发展 方法论。它将作为一个跨学科的平台 调查和培训，并将显著提高 MUSC调查人员的竞争力。

The purpose of this proposal is to obtain equipment to create an integrated Cellular Fluorescence Analysis Facility at the Medical University of South Carolina (MUSC). The key components of the proposed facility are a Fluorometric Laser Imaging Plate Reader (FLIPR) and a FACSCalibur Analytical Flow Cytometer. This facility is requested by a group of 11 major users in three colleges (Medicine, Dental Medicine, and Pharmaceutical Sciences) and 6 departments within the College of Medicine (Medicine, Microbiology, Pharmacology, Pathology, Physiology, and Experimental Oncology). The specific aims are as follows: 1. To obtain a Fluorometric Laser Imaging Plate Reader (FLIPR) and a FACSCalibur Analytical Flow Cytometer for the MUSC Cellular Fluorescence Analysis Facility. 2. To use this facility to enhance the productivity of NIH-funded investigators and to improve the research efficiency and competitiveness of MUSC research and trainees. 3. To use this facility to foster interdisciplinary work among the users. The facility addresses a broad need for flexible and high throughput fluorescence analyses to support research in many different disciplines. A detailed organizational plan, substantial institutional commitment of support, and a long term plan for maintenance ensures the sustained viability of the facility. The availability of the integrated Cellular Fluorescence Analysis Facility will provide unique opportunities for the development of novel new uses for cellular fluorescence detection methodologies. It will serve as a platform for interdisciplinary investigation and training, and will significantly enhance the competitiveness of MUSC investigators.