AUTOMATED DNA SEQUENCER

正在寻求资金购买一台自动DNA测序仪 以及相关的小型设备和软件，以准备和 分析DNA序列。该仪器将被放置在中央- 位于生物技术设施内，将提供给 研究应用包括(但不限于)的调查人员 到)分子进化和系统发育、鉴定和 新基因的鉴定和突变相关基因的研究 到表型表达。目前，主要用户和次要用户包括 在这项提议中，使用人工放射性同位素方法来产生DNA 序列数据。除了健康风险和高昂的成本外， 与放射性物质有关的废物处理，人工排序 方法严重阻碍了每一种方法的研究效率 调查人员。然而，自动测序会使健康失效。 风险，降低了DNA测序的总体成本，并增加了 与人工方法相比，吞吐量提高了压倒性的百分比。建议数 自动DNA测序机将安装在一个多用户 生物技术设施，以促进一致的 设备的维护、使用和融资。次要用户将 对每个测序的模板收取适当的费用 收回技术人员时间、耗材和 仪器维护。四大用户(即私家侦探S)，在 与生物技术设施主任的合作，将形成 成立督导委员会以厘定服务及编排时间的费用 并将可以选择在同一计算机上使用该计算机 按服务收费以及允许个人生成 测序模板并操作机器。副局长的办公室 医学院研究部主任兼研究部副院长 西北大学的学校一直致力于生物技术 关于机构空间的设施、设施主任和 技术员工资，以及拟议设备的持续维护。

Funds are being sought for the purchase of an automated DNA sequencer plus associated small equipment and software necessary to prepare and analyze DNA sequences. The instrument will be placed in a centrally- located Biotechnology facility and will be available to a community of investigators whose research applications include (but are not limited to) molecular evolution and phylogenetics, identification and characterization of new genes, and the study of mutant genes relative to phenotypic expression. Currently, the major and minor users included in this proposal employ manual radioisotopic methods to generate DNA sequence data. In addition to the health risks and exorbitant costs of waste disposal associated with radioactive material, manual sequencing methods are a severe hindrance to the research productivity of each of the investigators. Automated sequencing, however, annuls the health risks, lowers the overall cost of DNA sequencing and increases throughput by an overwhelming percent over manual methods. The proposed automated DNA sequencing machine will be housed in a multiple-user Biotechnology facility in order to facilitate the consistent maintenance, usage, and financing of the equipment. Minor users will be charged a fee for each sequenced template that is appropriate to recover the costs of technician time, consumable materials, and instrument maintenance. The four major users (i.e., the P.I.'s), in collaboration with the Director of the Biotechnology facility, will form a steering committee to determine the fee for service and scheduling of the machine and will have the option of using the machine on the same fee-for service basis as well as being allowed to individually generate sequencing templates and operate the machine. The offices of the Vice President for Research and Associate Dean for Research at the Medical School at Northwestern have an ongoing commitment to the Biotechnology facility with regard to institutional space, facility director and technician salary, and continued maintenance of the proposed equipment.