MULTIDEPARTMENTAL FLOW CYTOMETRY FACILITY

这一修订后的申请要求资金用于升级流式细胞仪 在一个既定的多部门设施内的仪器， 约翰霍普金斯医学院。具体来说，我们要求资金 升级我们目前的细胞分选机，双激光- FACStarplus状态 最先进的高速分拣能力。这一要求是由 对高速分拣能力的科学要求， 稀有和次要细胞亚群的分离。 该设施于1989年建立，部分资金来自一个 共享工具补助金。目前的仪器已被证明是 在推进用户的研究使命方面是非常宝贵的。但 越来越多的人需要分离稀有细胞群， 功能和分子分析。当前仪器的容量 对我们应对日益增长的需求的能力设置了实际限制 鉴定、分析和分离次要和稀有细胞亚群。 在这份提案中，7个主要研究小组描述了他们目前和 未来的研究活动，以及这些活动将如何受益于 要求升级为高速分拣。 多部门流式细胞仪设施有一个既定的计划， 自1989年以来一直运行，以确保长期访问，维护 和仪器的可用性。 该设施的工作人员是一个 全职经理/操作员，由设施总监和 咨询委员会。该设施每年通过混合资金供资， 使用者费用，中心和项目项目的同行评审资金， 机构资源。这一承诺将继续下去， 设施保持其提供“最先进”的能力 仪器，以满足流式细胞术的需要，霍普金斯生物医学 研究社区。

This revised application requests funds for upgrading the flow cytometry instrumentation within a established multi-departmental facility at the Johns Hopkins School of Medicine. Specifically, we are requesting funds to upgrade our current cell sorter, a dual laser - FACStarplus to state of the art high speed sorting capabilities. This request is driven by the scientific demand for high speed sorting capability specifically for the isolation of rare and minor cell subpopulations. The facility was established in 1989, in part, through the funding of a shared instrument grant. The current instrumentation has proven to be invaluable in forwarding the research mission of the users. However, there is increasing demand on the isolation of rare cell populations for functional and molecular analysis. The capacity of the current instrument sets a practical limit on our ability to respond to the increasing demand to identify, analyze and isolate minor and rare cell subpopulations. Within this proposal, 7 major research groups describe their current and future research activities and how these activities will benefit from the requested upgrade for high speed sorting. The multi-departmental flow cytometry facility has a established plan that has been operational since 1989 to ensure long term access, maintenance and availability of the instrumentation. The facility is staffed by a full-time manager/operator and is overseen by a facility director and a advisory committee. The facility has been annually funded through a blend of user fees, peer-reviewed funding of center and program projects and Institutional resources. This commitment will continue so that the facility maintains its ability to provide "state of the art" instrumentation to meet the flow cytometry needs of the Hopkins Biomedical Research community.