SPECTROSCOPIC SUITE

理想情况下，收购最先进的主要仪器设备 对不同学科的广泛科学家产生了影响。这个 匹兹堡大学在此建议配备一台岩心光谱仪 为科学界提供利用手段的设施 圆二色谱(CD)，以进一步开展各自的研究。 这些目标将通过购买AVIV型号62A型来实现 DS圆二色光谱仪配备了最先进的光学系统， 温度控制、注射器泵和荧光检测。一个 Macintosh计算机接口提供数据收集控制 在波长、时间或温度上进行分析，并允许进行简单的分析。 配套的高性能AVIV型14DS紫外-可见光谱仪 提供准确的相应吸光度分布。 圆二色谱是一种非常有用的方法来测定 蛋白质二级结构和/或稳定性。它是非破坏性的 朝向样品(即样品在光谱分析后被完全回收) 并且需要相对较少的提纯材料 (约100微克)。 这份提案描述了一系列目前受到限制的项目 由于匹兹堡地区没有这样的工具。研究 以及从分子发病机制到各种领域的教育活动 HIV在非水环境中对酶的化学工程的影响 受到CD光谱仪到来的重大影响。这个 分子遗传学和分子生物学系蛋白质核心设施 匹兹堡大学医学院的生物化学将 协调仪器的采购、维护和使用。一个 咨询委员会将确定公平分享 这一资源将向科学界开放， 推进各自的研究项目和领域。

The acquisition of major state of the art instrumentation should ideally impact on a wide range of scientists in various disciplines. The University of Pittsburgh proposes herein to equip a core spectroscopic facility to provide the scientific community with the means to utilize circular dichroism (CD) spectroscopy to further their respective research. These goals will b e realized through the purchase of an AVIV model 62A DS Circular Dichroism spectrometer equipped with state of the art optics, temperature control, syringe pumps, and fluorescence detection. A Macintosh computer interface provides for control of data collection over wavelength, time, or temperature, and allows for facile analyses. A companion high performance AVIV model 14DS UV-Vis Spectrophotometer provides for accurate corresponding absorbance profiles. CD spectroscopy is a highly useful methodology for the determination of protein secondary structure and/or stability. It is non-destructive towards the sample (i.e. samples are fully recovered after spectroscopy) and requires relatively low quantities of purified material (approximately 100 ug). This proposal describes an array of projects which are currently limited by the absence of such an instrument in the Pittsburgh region. Research and educational activities in fields as diverse as molecular pathogenesis of HIV to chemical engineering of enzymes in non-aqueous environments will be significantly impacted by the arrival of a CD spectrometer. The Protein Core facility of the Department of Molecular Genetics and Biochemistry at the University of Pittsburgh School of Medicine will coordinate the purchase, maintenance and use of the instrument. An advisory committee will determine guidelines for the equitable sharing of this resource which will be open to the scientific community for the advancement of their respective research projects and fields.