LEICA TCSNT CONFOCAL MICROSCOPE

此应用程序的目的是获取更新的共焦 波士顿大学医学院的显微镜 共焦显微镜核心设备。该工厂目前拥有一辆徕卡 1991年购买的共焦激光扫描系统。基金是 申请购买徕卡TCS NT共焦图像分析工作站 用倒置显微镜。将扫描仪放置在 立式显微镜也包括在内，但将由机构提供资金 支持。该软件包括3D和生理学程序作为几个 研究人员计划获取染色的活组织的延时记录 使用活性染料、钙或pH指示剂。目前《解剖学》和《纽约时报》 神经生物系已经为显微镜分配了空间， 医学院院长、研究生院长和系主任承诺 制度保障。机构支持将包括资金 购买额外的直立式显微镜和其他配件。广泛性 收集了1992-1996年当前用户组的数据。这个 现有的共焦系统每年使用超过800小时，需要数月 超过100小时。五个主要研究小组和八个次要研究小组 已确定将需要新设备用于广泛的 项目。研究人员跨越了许多年龄段，从年轻的研究人员开始 敬资深科学家。大多数参与者都有目前的小灵通赠款支持， 并期待着通过 为核心设施配备这种新显微镜的设备。我们目前的情况 机器只有6年的历史，但计算机系统已经过时， 简单的维修需要很长的时间。此外，它还受限于 可以看到的样品的类型，因为它只配备了Ar 激光器和两个光电倍增管。

The purpose of this application is to acquire an updated confocal microscope for the established Boston University School of Medicine Confocal Microscopy Core Facility. The facility currently houses a Leica confocal laser scanning system that was purchased in 1991. Funds are requested to purchase an Leica TCS NT confocal image analysis work station with an inverted microscope. The option of placing the scanner on an upright microscope is also included, but will be funded from institutional support. The software includes the 3D and physiology programs as several researchers plan to obtain time lapse recordings of live tissue stained with vital dyes, calcium or pH indicators. Currently the Anatomy and Neurobiology Department has allocated space for the microscope, and the Dean of Medicine, Graduate Dean, and Departmental Chairs have promised institutional support. The institutional support will include money to purchase an additional upright microscope and other accessories. Extensive data has been collected on the current user group from 1992-1996. The existing confocal system is used over 800 hrs/year with many months topping 100 hrs. Five primary and eight secondary researcher groups have been identified that will need the new equipment for a broad range of projects. The researchers span many age groups, from young investigators to senior scientists. Most participants have current PHS grant support, and are looking forward to enhancing and extending their research with the equipment with this new microscope for the core facility. Our current machine is only 6 years old, however, the computer system is obsolete and simple repairs take extensive time. In addition, it is limited in the types of specimens that can be viewed as it is equiped with only an argon laser and two photomultiplier tubes.