FOAMY VIRUS INFECTION OF PERIPHERAL BLOOD FIBROCYTES: LYME DISEASE

我们已经证明莱姆病螺旋体B。burgdorferi， 可以侵入外周血纤维细胞，CD 4-，CD 8-， CD 14-白细胞亚群，具有靶向结缔组织的潜力 纸巾，纤维细胞分泌I型胶原和 招募和激活T细胞表明它们在免疫系统中起着关键作用， 在某些结缔组织疾病中。 我们最近采集了 来自威廉姆斯医生的一名患者，该患者有记录的重度 疲劳综合征（根据CDC指南）以及EIA和Western blot B阳性。血液和脑脊液中的伯氏菌（IgG） 样品 因为病人可能接触到B。 我们研究了他们的纤维细胞， 螺旋体入侵 电子显微镜检查显示， 在病人的外周血中发现了螺旋体 血纤维细胞与正常人相比。 尽管 缺乏B的证据burgdorferi我们发现了甜甜圈形状的 纤维细胞内的逆转录病毒样颗粒， 空泡 在大的，扩张的脑池中发现了类病毒。 内质网，它渗透到整个细胞体，除了 细胞核周围的区域 这样的泡沫外观， 是泡沫病毒的特征 “泡沫”病毒高度 可以感染CD 14+单核细胞和CD 4 +/CD 8+的世界性病毒 白细胞，但它们在人类疾病中的作用仍然存在争议。 在这里，我们第一次展示了泡沫细胞对纤维细胞的感染。 病毒，但还有待观察，如果出现这种症状， 患者与泡沫状病毒样的存在有关

We have shown that the Lyme disease spirochete, B. burgdorferi, can invade peripheral blood fibrocytes, a CD4-, CD8-, and CD14-leukocyte sub population, with the potential to target connective tissue., The ability of fibrocytes to secrete type I collagen and to recruit and activate T cells suggests that they play a critical role in certain connective tissue disorders. We recently obtained blood from a patient of Dr. Williams with a history of documented severe fatigue syndrome (by CDC guidelines) as well as EIA and Western blot positivity for B. burgdorgeri (IgG) in blood and cerebral spinal fluid samples. Because of the patient's potential exposure to B. burgdorgeri we investigated their fibrocytes for evidence of spirochete invasion. Electron microscopic examination showed no evidence of spirochetes within this patient's peripheral blood-fibrocytes when compared to normal individuals. Despite the lack of evidence for B. burgdorferi, we found doughnut shaped, retrovirus-like particles within fibrocytes containing numerous vacuoles. Viroids were found in large, dilated cisternae of the endoplasmic reticulum which permeated the entire cell body except for the area around the nucleus. Such a foamy' appearance is characteristic of Spumaviruses. 'Foamy' viruses are highly cosmopolitan viruses which can infect CDl4+ monocytes, and CD4+/CD8+ leukocytes, but their role in human disease remains controversial. Here, we show for the first time the infection of fibrocytes by foamy' viruses, but it remains to be seen if presenting symptoms of this patient are associated with the presence of the foamy virus-like