GENETIC DIVERSITY OF SIMIAN RETROVIRUSES

猿猴D型逆转录病毒是猿猴获得性疾病的原因 亚洲生物医学研究社区中的免疫缺陷综合征 猕猴，并可以作为模型系统来理解 调控细胞趋向性的包膜糖蛋白基因多样性 并在艾滋病发病机制中起病。逆转录病毒经历高频 它们env基因的突变，并受到选择压力的影响 这导致了新的病理优势的出现 变种。我们已经进行了分子克隆和测序 D型猴逆转录病毒(SRV血清组2；D2/RHE/OR)基因组和 从地方性感染恒河猴中发现变异病毒 与典型的血清组2病毒有关。感染性分子基因组 D2/RHE/OR和一个变种D2/RHE/OR/V1已被恢复。喜欢 分子克隆的2型SRV(D2/CEL/OR)分离株 西里伯斯猕猴，恒河猴的env基因 逆转录病毒编码574个氨基酸的糖蛋白。Env基因 D2/RHE/OR和D2/CEL/OR在氨基酸水平上有96%的相似性； D2/RHE/OR和V1糖蛋白之间的相似程度为 98%(或5个残基转化率)。此外，我们已经分离出 血清组4(D4/Cyn/CA)和血清组5的感染性分子克隆 (D5/RHE/OR)SRV。完成这些附加的序列分析 基因组克隆表明，血清群4和5是SRV 在基因上有别于所有以前描述的血清群。 对血清组4和5的SRV的全序列分析 提供了有关该物种遗传多样性的重要信息 猿猴逆转录病毒，并将提供必要的分子试剂 用于未来的结构功能和细胞/组织趋向性实验。

The simian type D retroviruses are the cause of Simian Acquired Immunodeficiency Syndrome in biomedical research communities in Asian macaques, and can serve as model systems in understanding the role of envelope (env) glycoprotein gene diversity in modulating cell tropism and onset in AIDS pathogenesis. Retroviruses undergo high-frequency mutations in their env genes and are subjected to selection pressures that result in the appearance of new pathologically-advantaged variants. We have molecularly-cloned and sequenced the complete genomes of a type D simian retrovirus (SRV serogroup 2; D2/RHE/OR) and variant viruses recovered from rhesus macaques endemically infected with the prototypical serogroup 2 virus. Infectious molecular genomes of D2/RHE/OR and one variant, D2/RHE/OR/V1, have been recovered. Like the molecularly-cloned serogroup 2 SRV (D2/CEL/OR) isolated from the Celebes macaque, the env genes from the rhesus-derived simian retroviruses encode a glycoprotein of 574 amino acids. The env genes of D2/RHE/OR and D2/CEL/OR are 96% similar at the amino acid level; the degree of similarity between the D2/RHE/OR and V1 glycoproteins is 98% (or 5 residue conversions). In addition, we have isolated infectious molecular clones of serogroup 4 (D4/CYN/CA) and serogroup 5 (D5/RHE/OR) SRVs. Complete sequence analyses of these additional genomic clones indicate that serogroups 4 and 5 SRVs are genetically-distinct from all previously characterized serogroups. The complete sequence analyses of the serogroup 4 and 5 SRVs has provided important information concerning the genetic diversity of the simian retroviruses, and will provide the necessary molecular reagents for future structure-function and cell/tissue tropism experiments.