DIETARY APPROACHES TO STOP HYPERTENSION 2 (DASH 2)

DASH2中本辅助研究的目的是确定 膳食结构对血清脂质过氧化速率的影响 抗氧化活性和LDL-胆固醇氧化的指标，以及 来绘制它们的时间进程 DASH2试验提供了基础设施 为拟议的研究。 DASH2是一项喂养研究，旨在评估 2种膳食模式中3种盐水平对血压的影响 100名收缩压120 - 159 mmHg和舒张压120 - 159 mmHg的成人 压力80至95 mmHg。 在两周的时间里，参与者被喂食一种对照品， 饮食，低水果，蔬菜和乳制品，脂肪和盐 典型的美国消费。 然后，参与者被随机分配到 （1）对照饮食或（2）"组合"的平行臂设计 饮食丰富的水果，蔬菜和低脂乳制品，并减少 饱和脂肪和总脂肪含量。 在每一种饮食中，参与者都会收到 三种不同的钠水平（50 mmol/d、100 mmol/d和150 mmol/d）， 每种持续30天。 研究参与者将是100名健康志愿者， DASH2在约翰霍普金斯综合临床研究中心。 到目前为止， 我们收集了35名随机 参与者参加了三个喂养队列中的第一个。 采样 除了基线外，还将进行血液和尿液的检查。 测量（随机化前），在每30天结束时。 主要结果指标，即氧化损伤指标， 包括尿中异前列腺素和丙二醛以及血清抗体 氧化的LDL。 拟议中的研究有可能提高我们的基本认识 饮食影响这些重要媒介的机制 心血管疾病

The objective of this ancillary study in DASH2 is to determine the impact of dietary patterns on rates of lipid peroxidation, serum antioxidant activity, and indicators of LDL-cholesterol oxidation, and to chart their time course. The DASH2 trial provides the infrastructure for the proposed research. DASH2 is a feeding study designed to assess the effects of 3 salt levels in 2 dietary patterns on blood pressure in 100 adults with systolic blood pressure 120-159 mmHg and diastolic blood pressure 80 to 95 mmHg. For two weeks, participants are fed a control diet, low in fruits, vegetables, and dairy products, with fat and salt content typical of US consumption. Participants are then randomized in a parallel arm design to (1) the control diet or (2) a "combination" diet rich in fruits, vegetables, and low-fat dairy products, and reduced in saturated and total fat. Within each diet, participants receive three different sodium levels (50 mmol/d, 100 mmol/d, and 150 mmol/d), each for 30 days. Study participants will be the 100 healthy volunteers to be enrolled in DASH2 at the Johns Hopkins General Clinical Research Center. To date, we have collected blood and urine specimens from 35 randomized participants enrolled in the first of three feeding cohorts. Sampling of blood and urine will take place, in addition to the baseline measurement (prior to randomization), at the end of each 30 day period. The primary outcome measures, which are measures of oxidative damage, include urinary isoprostanes and malondialdehyde and serum antibodies to oxidized LDL. The proposed study has the potential to enhance our basic understanding of the mechanisms by which diet affects these important intermediaries of cardiovascular disease.